Polyhydroxyalkanoates (PHA) production by photoheterotrophic microbial consortia: Effect of culture conditions over microbial population and biopolymer yield and composition

被引:41
作者
Guerra-Blanco, P. [1 ,2 ]
Cortes, O. [1 ]
Poznyak, T. [2 ]
Chairez, I. [1 ]
Garcia-Pena, E. I. [1 ]
机构
[1] Inst Politecn Nacl, Bioproc Dept, Unidad Profes Interdisciplinaria Biotecnol, Mexico City 07340, DF, Mexico
[2] Inst Politecn Nacl, Escuela Super Ingn Quirn & Ind Extract, UPALM, Edif 7, Mexico City 07738, DF, Mexico
关键词
Photoheterotrophic; Mixed microbial consortium; Polyhydroxyalkanoates (PHAs); Poly(3-hydroxybutyrate) (P3HB); Poly(3-hydroxyvalerate) (P3HV); MILL WASTE-WATER; ACTIVATED-SLUDGE; FATTY-ACIDS; BACTERIAL POLYHYDROXYALKANOATE; BIODEGRADABLE POLYESTERS; POLYHYDROXYBUTYRATE PHB; PSEUDOMONAS-OLEOVORANS; BETA-HYDROXYBUTYRATE; ANAEROBIC OXIDATION; THERMAL-PROPERTIES;
D O I
10.1016/j.eurpolymj.2017.11.007
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Three microbial consortia (C2, C4, C5) grown under photoheterotrophic conditions assimilated acetate and butyrate, as individual and mixed substrates. By controlling the culture conditions, it was possible to manipulate the microbial population composition and thus the yield of polyhydroxyalkanoates (PHA) accumulation. Under limited ammonium conditions, pH control, and a sequential two-step process, C2 and C4 produced PHA. C4 showed the highest production of 44% of the cell dry mass (CDM), close to the theoretical value calculated with a stoichiometric balance. Analysis of the confocal microscopy images confirmed the accumulated biopolymer percentages produced by each consortium, and it was in close correlation with microbial distribution and substrate consumption pattern. H-1, C-13, NMR, and MALDI-TOF spectra identified the primary structure of the obtained biopolymers as copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV). This composition allows for better mechanical properties compared to the PHB homopolymer. Microbial characterization showed a similar microbial population with different proportions for C2 and C4. The highest PHA production in C4 was associated with higher abundances of PHA producers, including Clostridium (29%), Pseudomonas (8%) and Rhodopseudomonas (5%). Both microbial consortia showed that a portion of their microbial populations were able to perform syntrophic reactions (Dysgonomonas and Clostridium).
引用
收藏
页码:94 / 104
页数:11
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