Assessing the Fecal Microbiota: An Optimized Ion Torrent 16S rRNA Gene-Based Analysis Protocol

被引：247

作者：

Milani, Christian ^{[1
]}

Hevia, Arancha ^{[2
]}

Foroni, Elena ^{[1
]}

Duranti, Sabrina ^{[1
]}

Turroni, Francesca ^{[3
,4
]}

Lugli, Gabriele Andrea ^{[1
]}

Sanchez, Borja ^{[2
]}

Martin, Rebeca ^{[5
]}

Gueimonde, Miguel ^{[2
]}

van Sinderen, Douwe ^{[3
,4
]}

Margolles, Abelardo ^{[2
]}

Ventura, Marco ^{[1
]}

机构：

[1] Univ Parma, Lab Probiogen, Dept Life Sci, I-43100 Parma, Italy

[2] IPLA CSIC, Dept Microbiol & Bioquim Prod Lacteos, Villaviciosa, Asturias, Spain

[3] Natl Univ Ireland Univ Coll Cork, Alimentary Pharmabiot Ctr, Biosci Inst, Cork, Ireland

[4] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Biosci Inst, Cork, Ireland

[5] MICALIS Microbiol Lalimentat Serv Sante humaine, INRA, UMR 1319, F-78352 Jouy En Josas, France

来源：

PLOS ONE | 2013年 / 8卷 / 07期

基金：

爱尔兰科学基金会;

关键词：

GUT MICROBIOTA; PHYLOGENETIC MICROARRAY; INTESTINAL MICROBIOTA; BACTERIAL; DIVERSITY; COMMUNITIES; SEQUENCE; SUCCESSION; HEALTH; IMPACT;

D O I：

10.1371/journal.pone.0068739

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.

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页数：12

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