Regulated Expression of PTPRJ/CD148 and an Antisense Long Noncoding RNA in Macrophages by Proinflammatory Stimuli

被引：37

作者：

Dave, Richa K. ^{[1
,2
,3
]}

Dinger, Marcel E. ^{[4
,5
]}

Andrew, Megan ^{[3
]}

Askarian-Amiri, Marjan ^{[1
]}

Hume, David A. ^{[1
,2
,6
]}

Kellie, Stuart ^{[1
,2
,3
]}

机构：

[1] Univ Queensland, Inst Mol Biosci, Brisbane, Qld, Australia

[2] Univ Queensland, CRC CID, Brisbane, Qld, Australia

[3] Univ Queensland, Australian Infect Dis Res Ctr, Sch Chem & Mol Biosci, Brisbane, Qld, Australia

[4] Univ Queensland, Diamantina Inst, Brisbane, Qld, Australia

[5] St Vincents Hosp, Garvan Inst Med Res, Darlinghurst, NSW 2010, Australia

[6] Univ Edinburgh, Roslin Inst, Roslin, Midlothian, Scotland

来源：

PLOS ONE | 2013年 / 8卷 / 06期

基金：

澳大利亚国家健康与医学研究理事会;

关键词：

PROTEIN-TYROSINE-PHOSPHATASE; PLASMINOGEN-ACTIVATOR GENE; TRANSCRIPTION FACTORS; INTERFERON-GAMMA; CELL; RECEPTOR; CD148; CSF-1; CANCER; GROWTH;

D O I：

10.1371/journal.pone.0068306

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

PTPRJ/CD148 is a tyrosine phosphatase that has tumour suppressor-like activity. Quantitative PCR of various cells and tissues revealed that it is preferentially expressed in macrophage-enriched tissues. Within lymphoid tissues immunohistochemistry revealed that PTPRJ/CD148 co-localised with F4/80, indicating that macrophages most strongly express the protein. Macrophages express the highest basal level of ptprj, and this is elevated further by treatment with LPS and other Toll-like receptor ligands. In contrast, CSF-1 treatment reduced basal and stimulated Ptprj expression in human and mouse cells, and interferon also repressed Ptprj expression. We identified a 1006 nucleotide long noncoding RNA species, Ptprj-as1 that is transcribed antisense to Ptprj. Ptprj-as1 was highly expressed in macrophage-enriched tissue and was transiently induced by Toll-like receptor ligands with a similar time course to Ptprj. Finally, putative transcription factor binding sites in the promoter region of Ptprj were identified.

引用

页数：13

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