INFLUENCE OF VARIOUS PHYSICAL PARAMETERS ON ANTHER CULTURE OF BARLEY

被引：3
|
作者：
Guasmi, Ferdaous [1 ]
Elfalleh, Walid [1 ]
Hannachi, Hedia [1 ]
Feres, Khadija [1 ]
Touil, Leila [1 ]
Marzougui, Nidhal [1 ]
Triki, Tebra [1 ]
Ferchichi, Ali [1 ]
机构：
[1] Inst Reg Arides Medenine, Lab Aridoculture & Cultures Oasiennes, Medenine 4119, Tunisia
来源：
JOURNAL OF PLANT NUTRITION | 2013年 / 36卷 / 05期
关键词：
anther; barley; green plant; anther pretreatment; stage of microspores; Hordeum vulgare L; DOUBLED HAPLOID PLANTS; REGENERATION; EMBRYOGENESIS; ANDROGENESIS; PRETREATMENT; SORBITOL; CALLUS; ACID;
D O I：
10.1080/01904167.2012.759230
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
This study describes the effect of some parameters on anther culture of barley to optimize the plant regeneration condition of this species. The embryo formation and plant regeneration from anthers of three barley cultivars (Igri', Saida' and Libya') were investigated. The effects of length of flag leaves, stage of microspore development, and pre-treatments (mannitol or cold pre-treatment) of anthers were investigated. Results showed significant responses. Anthers at the mid uninucleate to mid-late uninucleate stage gave the best anther culture response showing 80, 60, and 30% anther development in Igri', Saida' and Libya' barley cultivars, respectively. The use of mannitol (0.3M) or 20days cold pre-treatments showed the best results for embryo and green-plant production.
引用
收藏
页码：836 / 847
页数：12
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