Antineoplasic activity of Copaifera multijuga oil and fractions against ascitic and solid Ehrlich tumor

被引:45
作者
Gomes, Niele de Matos [1 ]
Rezende, Claudia de Moraes [2 ]
Fontes, Silvia Paredes [2 ]
Hovell, Ana Maria Celestino [2 ]
Landgraf, Richardt Gama [3 ]
Matheus, Maria Eline [1 ]
Pinto, Angelo da Cunha [2 ]
Fernandes, Patricia Dias [1 ]
机构
[1] Univ Fed Rio de Janeiro, Programa Desenvolvimento Farm, ICB, BR-21944970 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Quim, Ctr Tecnol, BR-21941 Rio De Janeiro, Brazil
[3] Univ Sao Paulo, ICB, Dept Imunol, BR-05508 Sao Paulo, Brazil
关键词
Copaifera multijuga; Ehrlich ascitic tumor; cancer; copaiba;
D O I
10.1016/j.jep.2008.06.033
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The aim of this study was to investigate the effect of chronic treatment with C. multijuga oil on Ehrlich tumor evolution. C multijuga was fractionated in a KOH impregnated silica gel column chromatography to give three distinct fractions, i.e., hexanic, chloroformic, and methanolic, mainly composed by hydrocarbon sesquiterpenes, oxygenated sesquiterpenes and acidic diterpenes, respectively. Results demonstrated that the C multijuga oil, the hexanic, and chloroformic fractions did not develop toxic effects. The oil, hexanic and chloroformic fractions (doses varying between 100 and 200 mg/kg) showed antineoplasic properties against Ehrlich ascitic tumor (EAT) and solid tumor during 10 consecutive days of treatment inhibiting ascitic tumor cell number, reverting medulla and blood cell counts to values similar to control group, and inhibiting the increase on several inflammatory mediators (total protein, PGE(2), nitric oxide, and TNF) on ascitic fluid. The treatment also inhibited the increase in paw volume on tumor-inoculated mice. In conclusion, C. multijugo as well as its fractions demonstrated antineoplasic effect even after oral administration confirming its use by traditional medicine. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:179 / 184
页数:6
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