Self-assembled monolayer for low density lipoprotein detection

被引：13

作者：

Matharu, Zimple ^{[1
,2
]}

Arya, Sunil K. ^{[1
]}

Sumana, G. ^{[1
]}

Gupta, Vinay ^{[2
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Univ Delhi, Dept Phys & Astrophys, Delhi 110007, India

来源：

JOURNAL OF MOLECULAR RECOGNITION | 2008年 / 21卷 / 06期

关键词：

self-assembled monolayer; surface plasmon resonance; heparin; low density lipoprotein;

D O I：

10.1002/jmr.922

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Heparin (HEP) has been covalently immobilized onto 4-aminothiophenol (ATIP) self-assembled monolayer (SAM) deposited onto gold (Au)-coated glass plate for low density lipoprotein (LDL) detection. The HEP/ATP/Au and LDL/ HEP/ATP/Au electrodes have been characterized using cyclic voltammetry (CV) and scanning electron microscopy (SEM). Surface plasmon resonance (SPIR) measurements reveal that HEP/ATP/Au electrode is sensitive to detection of the LDL in the range 0.03 mu M (10mg/dl)-0.39 mu M (130mg/dl). The values of association and dissociation rate constants in the association phase calculated by kinetic analysis have been found to be k(a) = 9.67 X 10(1) M-1 s(-1) and k(d) = 2.64 X 10(-4) s(-1). Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：419 / 424

页数：6

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