Immunomagnetic separation of B type acute lymphoblastic leukemia cells from bone marrow with flow cytometry validation and microfluidic chip measurements

被引：6

作者：

Icoz, Kutay ^{[1
,2
]}

Eken, Ahmet ^{[3
]}

Cinar, Suzan ^{[4
]}

Murat, Aysegul ^{[3
]}

Ozcan, Servet ^{[3
,5
]}

Unal, Ekrem ^{[3
,6
]}

Deniz, Gunnur ^{[4
]}

机构：

[1] Abdullah Gul Univ, BioMINDS Bio Micro Nano Devices & Sensors Lab, Dept Elect & Elect Engn, Kayseri, Turkey

[2] Abdullah Gul Univ, Bioengn Dept, Kayseri, Turkey

[3] Erciyes Univ, Genome & Stem Cell Ctr GENKOK, Kayseri, Turkey

[4] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Immunol, Istanbul, Turkey

[5] Erciyes Univ, Biol Dept, Kayseri, Turkey

[6] Erciyes Univ, Pediat Hematol & Oncol Dept, Kayseri, Turkey

来源：

SEPARATION SCIENCE AND TECHNOLOGY | 2021年 / 56卷 / 15期

关键词：

B type acute lymphoblastic leukemia; immunomagnetic separation; flow cytometry; bone marrow; hematogone; microfluidic chip; MINIMAL RESIDUAL DISEASE; CONCURRENT DETECTION; ONCOLOGY-GROUP; MRD;

D O I：

10.1080/01496395.2020.1835983

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In order to detect the blast cells from bone marrow of patients, one strategy is to first isolate the cells using immunomagnetic beads. The aim of this study was to report the experimental results of the immunomagnetic separation efficiency of the blast cells from bone marrow of pediatric leukemia patients. To test the efficiency of immunomagnetic separation, flow cytometry measurements at critical steps were performed. We here reported 94.5% capture efficiency for CD10 nano beads. Patients samples were also analyzed with a microfluidic chip to test the feasibility for further developments.

引用

页码：2659 / 2666

页数：8

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