Immunomagnetic separation of B type acute lymphoblastic leukemia cells from bone marrow with flow cytometry validation and microfluidic chip measurements

被引：5
|
作者：
Icoz, Kutay [1 ,2 ]
Eken, Ahmet [3 ]
Cinar, Suzan [4 ]
Murat, Aysegul [3 ]
Ozcan, Servet [3 ,5 ]
Unal, Ekrem [3 ,6 ]
Deniz, Gunnur [4 ]
机构：
[1] Abdullah Gul Univ, BioMINDS Bio Micro Nano Devices & Sensors Lab, Dept Elect & Elect Engn, Kayseri, Turkey
[2] Abdullah Gul Univ, Bioengn Dept, Kayseri, Turkey
[3] Erciyes Univ, Genome & Stem Cell Ctr GENKOK, Kayseri, Turkey
[4] Istanbul Univ, Aziz Sancar Inst Expt Med, Dept Immunol, Istanbul, Turkey
[5] Erciyes Univ, Biol Dept, Kayseri, Turkey
[6] Erciyes Univ, Pediat Hematol & Oncol Dept, Kayseri, Turkey
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 2021年 / 56卷 / 15期
关键词：
B type acute lymphoblastic leukemia; immunomagnetic separation; flow cytometry; bone marrow; hematogone; microfluidic chip; MINIMAL RESIDUAL DISEASE; CONCURRENT DETECTION; ONCOLOGY-GROUP; MRD;
D O I：
10.1080/01496395.2020.1835983
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
In order to detect the blast cells from bone marrow of patients, one strategy is to first isolate the cells using immunomagnetic beads. The aim of this study was to report the experimental results of the immunomagnetic separation efficiency of the blast cells from bone marrow of pediatric leukemia patients. To test the efficiency of immunomagnetic separation, flow cytometry measurements at critical steps were performed. We here reported 94.5% capture efficiency for CD10 nano beads. Patients samples were also analyzed with a microfluidic chip to test the feasibility for further developments.
引用
收藏
页码：2659 / 2666
页数：8
相关论文
共 50 条
  • [1] Standardized flow cytometry for highly sensitive MRD measurements in B-cell acute lymphoblastic leukemia
    Theunissen, Prisca
    Mejstrikova, Ester
    Sedek, Lukasz
    van der Sluijs-Gelling, Alita J.
    Gaipa, Giuseppe
    Bartels, Marius
    da Costa, Elaine Sobral
    Kotrova, Michaela
    Novakova, Michaela
    Sonneveld, Edwin
    Buracchi, Chiara
    Bonaccorso, Paola
    Oliveira, Elen
    te Marvelde, Jeroen G.
    Szczepanski, Tomasz
    Lhermitte, Ludovic
    Hrusak, Ondrej
    Lecrevisse, Quentin
    Emilia Grigore, Georgiana
    Fronkova, Eva
    Trka, Jan
    Brueggemann, Monika
    Orfao, Alberto
    van Dongen, Jacques J. M.
    van der Velden, Vincent H. J.
    BLOOD, 2017, 129 (03) : 347 - 357
  • [2] Microfluidic Affinity Selection of B-Lineage Cells from Peripheral Blood for Minimal Residual Disease Monitoring in Pediatric B-Type Acute Lymphoblastic Leukemia Patients
    Witek, Malgorzata A.
    Larkey, Nicholas E.
    Bartakova, Alena
    Hupert, Mateusz L.
    Mog, Shalee
    Cronin, Jami K.
    Vun, Judy
    August, Keith J.
    Soper, Steven A.
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (19)
  • [3] Regeneration pattern of precursor-B-cells in bone marrow of acute lymphoblastic leukemia patients depends on the type of preceding chemotherapy
    EG van Lochem
    YM Wiegers
    R van den Beemd
    K Hählen
    JJM van Dongen
    H Hooijkaas
    Leukemia, 2000, 14 : 688 - 695
  • [4] Regeneration pattern of precursor-B-cells in bone marrow of acute lymphoblastic leukemia patients depends on the type of preceding chemotherapy
    van Lochem, EG
    Wiegers, YM
    van den Beemd, R
    Hählen, K
    van Dongen, JJM
    Hooijkaas, H
    LEUKEMIA, 2000, 14 (04) : 688 - 695
  • [5] A Novel Method for the Evaluation of Bone Marrow Samples from Patients with Pediatric B-Cell Acute Lymphoblastic Leukemia-Multidimensional Flow Cytometry
    Karai, Bettina
    Tisza, Katalin
    Eperjesi, Orsolya
    Nagy, Attila Csaba
    Ujfalusi, Aniko
    Kelemen, Agnes
    Szegedi, Istvan
    Kiss, Csongor
    Kappelmayer, Janos
    Hevessy, Zsuzsanna
    CANCERS, 2021, 13 (20)
  • [6] Major impact of an early bone marrow checkpoint (day 21) for minimal residual disease in flow cytometry in childhood acute lymphoblastic leukemia
    Eveillard, Marion
    Robillard, Nelly
    Arnoux, Isabelle
    Garand, Richard
    Rialland, Fanny
    Thomas, Caroline
    Strullu, Marion
    Michel, Gerard
    Bene, Marie C.
    Fossat, Chantal
    Loosveld, Marie
    HEMATOLOGICAL ONCOLOGY, 2017, 35 (02) : 237 - 243
  • [7] Sensitivity of combined DNA/immunophenotype flow cytometry for the detection of low levels of aneuploid lymphoblastic leukemia cells in bone marrow
    Nowak, R
    Oelschlaegel, U
    Schuler, U
    Zengler, H
    Hofmann, R
    Ehninger, G
    Andreeff, M
    CYTOMETRY, 1997, 30 (01): : 47 - 53
  • [8] Prognostic Value of Persistent Peripheral Blood and Bone Marrow Lymphoblasts on Day 15 of Therapy in Childhood Acute Lymphoblastic Leukemia as Detected by Flow Cytometry
    Jaworska-Posadzy, Anna
    Styczynski, Jan
    Kubicka, Malgorzata
    Debski, Robert
    Rafinska-Kurylo, Beata
    Kolodziej, Beata
    Pogorzala, Monika
    Wysocki, Mariusz
    ANTICANCER RESEARCH, 2011, 31 (04) : 1453 - 1457
  • [9] Interactions between acute lymphoblastic leukemia and bone marrow stromal cells influence response to therapy
    Tesfai, Yordanos
    Ford, Jette
    Carter, Kim W.
    Firth, Martin J.
    O'Leary, Rebecca A.
    Gottardo, Nicholas G.
    Cole, Catherine
    Kees, Ursula R.
    LEUKEMIA RESEARCH, 2012, 36 (03) : 299 - 306
  • [10] DETECTION OF ANEUPLOID CELLS IN ACUTE LYMPHOBLASTIC-LEUKEMIA WITH FLOW-CYTOMETRY BEFORE AND AFTER THERAPY
    NOWAK, R
    OELSCHLAGEL, U
    HOFMANN, R
    ZENGLER, H
    HUHN, R
    LEUKEMIA RESEARCH, 1994, 18 (12) : 897 - 901
12345