Ethanol up-regulates phenol sulfotransferase (SULT1A1) and hydroxysteroid sulfotransferase (SULT2A1) in rat liver and intestine

被引:16
|
作者
Maiti, Smarajit [1 ,2 ]
Chen, Guangping [1 ]
机构
[1] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Physiol Sci, Stillwater, OK 74078 USA
[2] Agricure Biotech Res Soc, Midnapore, W Bengal, India
关键词
Ethanol regulation; Hep G2 cells; protein expression; rat liver and intestine; sulfotransferases; HUMAN CYTOSOLIC SULFOTRANSFERASES; INDUCIBLE STEROID SULFOTRANSFERASE; X-RECEPTOR PXR; RETINOIC ACID; ALCOHOL-CONSUMPTION; NUCLEAR RECEPTOR; IN-VITRO; DEHYDROEPIANDROSTERONE SULFOTRANSFERASE; METABOLIC SYNDROME; BRASSICA-NAPUS;
D O I
10.3109/13813455.2014.992440
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ethanol-consumption impairs physiological-efficiency/endurance, expedites senescence. Impaired-regulations of steroids/biomolecules link these processes. Steroids are catabolized by cytosolic-sulfotransferases (SULTs). Ethanol-induction of eukaryotic-SULTs-expression is scanty. Plant (Brassica-napus) steroid-sulfotransferase; BNST3/BNST4 (gene/BNST) is highly ethanol-inducible (protein/mRNA). Resembling mammalian-SULTs catalytic-mechanism BNSTs show broad substrate-specificities (mammalian-steroids; estradiol/dehydroepiandrosterone/pregnanolone). Recently, ethanol-regulation of SULTs-expression is verified in rat liver/intestine/cultured human-hepatocarcinoma (Hep-G2) cells at enzyme-activity/protein-expression (Western-blot) level. Here, two week's ethanol ingestion by male rat significantly increased SULT2A1 in their liver/intestine (p<0.05-p<0.001) and phenol-sulfotransferase (SULT1A1) in intestine (p<0.001) at enzyme-activity/protein levels. In human cells, ethanol significantly (2-fold) increased hSULT1A1/hSULT1E (2-3 fold) protein expressions paralleling their enzymatic-activities (p<0.05-p<0.01). The earlier finding of alcohol-association to the physiological impairment may be corroborated by our present findings. Inductions of SULT-expressions by ethanol have significant physiological/pharmacological consequences.
引用
收藏
页码:68 / 74
页数:7
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