Toll-like Receptor agonists and alpha-galactosylceramide synergistically enhance the production of interferon-gamma in murine splenocytes

被引：19

作者：

Ando, Tatsuya ^{[1
]}

Ito, Hiroyasu ^{[1
]}

Ohtaki, Hirofumi ^{[1
]}

Seishima, Mitsuru ^{[1
]}

机构：

[1] Gifu Univ, Grad Sch Med, Dept Informat Clin Med, Gifu 5011194, Japan

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

NITRIC-OXIDE PRODUCTION; V-ALPHA-14 NKT CELLS; HEPATITIS-B-VACCINE; PHASE-I/II; TUMOR VACCINE; MELANOMA; ADJUVANT; IMMUNOGENICITY; IMMUNIZATION; PEPTIDE;

D O I：

10.1038/srep02559

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

V alpha 14 natural killer T (iNKT) cells activated by alpha-galactosylceramide (GalCer) secrete a large amount of cytokines. Toll-like receptors (TLRs) play a critical role in the innate immune responses via the recognition of pathological antigen. Previously we demonstrated that the iNKT cells activated by GalCer augmented LPS-induced NO production in peritoneal cells. In this study, we examined the effect of GalCer and TLR agonists by IFN-gamma production from splenocytes. Splenocytes pretreated with GalCer induced TLR3, 4, 7/8, and 9 agonists in vitro, resulting in the enhancement of IFN-gamma mRNA expression. In particular, IFN-gamma stimulated by GalCer and LPS was increased in NK cells and CD8 T cells, and inhibited by a neutralizing anti-IL-12 antibody. Pretreatment with GalCer enhanced the phosphorylation of I kappa B-alpha induced by LPS stimulation. The present study showed that co-stimulation of GalCer and TLR agonists powerfully induced the production of IFN-gamma from splenocytes.

引用

页数：6

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