SPECTROPHOTOMETRIC DETERMINATION OF DRUGS & PHARMACEUTICALS USING N-BROMOSUCCINIMIDE AND RHODAMINE-B COUPLE

Simple, sensitive, and accurate spectrophotometry method one each for estimation of five drugs viz., chloroquine phosphate (CHP), granisetron hydrochloride (GRA), rizatriptan benzoate (RIZ), zoledronic acid (ZOL) and zolmitriptan (ZOT), have been developed. The method depends upon oxidation of each drug by excess N-Bromo succinimide (NS), and subsequent determination of UN reacted NBS by Rhodamine-B dye at lambda(max), 557 nm. Beers law is obeyed between 2-14 mu g mL(-1) (for CHP); 52-14 mu g mL(-1) (for GRA); 1-7 mu g mL(-1) (for RIZ); 1-7 mu g mL(-1) (for ZOL) & 2-14 mu g mL(-1) (for ZOT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. All the methods have been validated as per ICH guidelines.