Photo-thermal effect enhances the efficiency of radiotherapy using Arg-Gly-Asp peptides-conjugated gold nanorods that target αvβ3 in melanoma cancer cells

被引：55

作者：

Li, Ping ^{[1
,3
]}

Shi, Yi-wen ^{[2
]}

Li, Bing-xin ^{[3
]}

Xu, Wen-cai ^{[4
]}

Shi, Ze-liang ^{[3
]}

Zhou, Chuanqing ^{[2
]}

Fu, Shen ^{[1
,3
]}

机构：

[1] Fudan Univ, Dept Radiat Oncol, Shanghai Proton & Heavy Ion Ctr, Canc Hosp, Shanghai 200433, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200030, Peoples R China

[3] Jiao Tong Univ, Peoples Hosp 6, Dept Radiat Oncol, Shanghai 200030, Peoples R China

[4] Zhengzhou Univ, Henan Canc Ctr, Dept Radiat Oncol, Zhengzhou 450052, Henan, Peoples R China

来源：

JOURNAL OF NANOBIOTECHNOLOGY | 2015年 / 13卷

基金：

中国国家自然科学基金;

关键词：

Arg-Gly-Asp peptides; Gold nanorods; Integrin alpha v beta 3; Photo-thermal therapy; Radiotherapy; BREAST-CANCER; HYPERTHERMIA; THERAPY; RADIATION; TUMORS;

D O I：

10.1186/s12951-015-0113-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: Thermotherapy has been known to be one of the most effective adjuvants to radiotherapy (RT) in cancer treatment, but it is not widely implemented clinically due to some limitations, such as, inadequate temperature concentrations to the tumor tissue, nonspecific and non-uniform distribution of heat. So we constructed arginine-glycine-aspartate peptides-conjugated gold nanorods (RGD-GNRs) that target the alpha(v) beta(3) Integrin (alpha v beta 3) and investigate whether the photo-thermal effect of RGD-GNRs by near infrared radiation (NIR) could enhance the efficiency of RT in melanoma cancer cells. Results: RGD-GNRs could be seen both on the surface of the cell membranes and cytoplasm of A375 cells with high expression of alpha v beta 3. After exposed to 808 nm NIR, RGD-GNRs with various concentrations could be rapidly heated up. Compared to other treatments, flow cytometric analysis indicated that RT + NIR + RGD-GNRs increased apoptosis (p < 0.001) and decreased the proportion of cells in the more radioresistant S phase (p = 0.014). Treated with NIR + RGD-GNRs, the radiosensitivity was also significantly enhanced (DMFSF2: 1.41). Conclusion: Results of the current study showed the feasibility of using RGD-GNRs for synergetic RT with photothermal therapy. And it would greatly benefit the therapeutic effects of refractory or recurrent malignant cancers.

引用

页数：8