Retinoic acid induces neurite outgrowth and growth cone turning in invertebrate neurons

被引:58
作者
Dmetrichuk, Jennifer M. [1 ]
Carlone, Robert L. [1 ]
Spencer, Gaynor E. [1 ]
机构
[1] Brock Univ, Dept Biol, St Catharines, ON L2S 3A1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
regeneration; electrophysiology; Lymnaea stagnalis; invertebrate; retinoids; electrical excitability; trophic factors; chemotaxis; neurite outgrowth; cell culture;
D O I
10.1016/j.ydbio.2006.02.018
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Identification of molecules involved in neurite outgrowth during development and/or regeneration is a major goal in the field of neuroscience. Retinoic acid (RA) is a biologically important metabolite of vitamin A that acts as a trophic factor and has been implicated in neurite outgrowth and regeneration in many vertebrate species. Although abundant in the CNS of many vertebrates, the precise role of RA in neural regeneration has yet to be determined. Moreover, very little information is available regarding the role of RA in invertebrate nervous systems. Here, we demonstrate for the first time that RA induces neurite outgrowth from invertebrate neurons. Using individually identified neurons isolated from the CNS of Lymnaea stagnalis, we demonstrated that a significantly greater proportion of cells produced neurite outgrowth in RA. RA also extended the duration of time that cells remained electrically excitable in vitro, and we showed that exogenously applied RA acted as a chemoattractive factor and induced growth cone turning toward the source of RA. This is the first demonstration that RA can induce turning of an individual growth cone. These data strongly suggest that the actions of RA on neurite outgrowth and cell survival are highly conserved across species. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:39 / 49
页数:11
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