Detection of Aichi virus with antibody targeting of conserved viral protein 1 epitope

被引:13
作者
Chen, Yao-Shen [1 ,2 ]
Chen, Bao-Chen [2 ]
Lin, You-Sheng [3 ,4 ]
Chang, Jenn-Tzong [3 ,4 ,5 ]
Huang, Tsi-Shu [2 ]
Chen, Jih-Jung [6 ]
Chang, Tsung-Hsien [4 ]
机构
[1] Kaohsiung Vet Gen Hosp, Dept Infect Dis, Kaohsiung 813, Taiwan
[2] Kaohsiung Vet Gen Hosp, Dept Microbiol, Kaohsiung 813, Taiwan
[3] Natl Sun Yat Sen Univ, Dept Biol Sci, Kaohsiung 804, Taiwan
[4] Kaohsiung Vet Gen Hosp, Dept Med Educ & Res, Kaohsiung 813, Taiwan
[5] Kaohsiung Vet Gen Hosp, Dept Pediat, Kaohsiung 813, Taiwan
[6] Tajen Univ, Grad Inst Pharmaceut Technol, Pingtung 907, Taiwan
关键词
VP1; Aichi virus; Kobuvirus; Picornaviridae; SEROPREVALENCE; IDENTIFICATION; REPLICATION; POPULATION; INFECTION; ASSAY;
D O I
10.1007/s00253-012-4644-5
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aichi virus (AiV) is an emerging single-stranded, positive-sense, non-enveloped RNA virus in the Picornaviridae that causes acute gastroenteritis in humans. The first case of AiV infection in Taiwan was diagnosed in a human neonate with enterovirus-associated symptoms; the virus was successfully isolated and propagated. To establish a method to detect AiV, we analyzed the antigen epitope and generated a polyclonal antibody against AiV viral protein 1 (VP1). This peptide-purified anti-AiV VP1 antibody showed high specificity against AiV VP1 without cross-reaction to nine other tested strains of Picornaviruses. The anti-AiV VP1 antibody was used in immunofluorescence analysis, immunoblotting, and enzyme-linked immunosorbent assay to elucidate the cell tropism and replication kinetics of AiV. Use of the anti-AiV VP1 antibody also revealed AiV infection restriction with interferon type I and polyI/C antiviral treatment. The AiV infection and detection system may provide an in vitro platform for AiV virology study.
引用
收藏
页码:8529 / 8536
页数:8
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