A protein-polymer hybrid gene carrier based on thermophilic histone and polyethylenimine

被引：5

作者：

Shi, Hui ^{[1
]}

Han, Haobo ^{[1
]}

Xing, Zhen ^{[1
]}

Chen, Jiawen ^{[1
]}

Wang, Yudi ^{[1
]}

Zhang, Aijun ^{[1
]}

Shi, Wei ^{[1
]}

Li, Quanshun ^{[1
]}

机构：

[1] Jilin Univ, Key Lab Mol Enzymol & Engn, Minist Educ, Sch Life Sci, Changchun 130012, Peoples R China

来源：

NEW JOURNAL OF CHEMISTRY | 2015年 / 39卷 / 09期

关键词：

IN-VITRO; DELIVERY; DNA; VECTOR; TRANSFECTION; COMPLEX; CELLS; VIVO; H3; HU;

D O I：

10.1039/c5nj01272d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A protein-polymer hybrid gene carrier HEP was successfully constructed based on thermophilic histone and polyethylenimine. The carrier exhibited low cytotoxicity and a high transfection efficiency due to the synergistic effects between the two components, which makes it a potential vehicle in tumor gene therapy.

引用

收藏

页码：6718 / 6721

页数：4

相关论文

共 26 条

[1] Histone H2A-mediated transient cytokine gene delivery induces efficient antitumor responses in murine neuroblastoma [J].

Balicki, D ;

Reisfeld, RA ;

Pertl, U ;

Beutler, E ;

Lode, HN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (21) :11500-11504

[2] A VERSATILE VECTOR FOR GENE AND OLIGONUCLEOTIDE TRANSFER INTO CELLS IN CULTURE AND IN-VIVO - POLYETHYLENIMINE [J].

BOUSSIF, O ;

LEZOUALCH, F ;

ZANTA, MA ;

MERGNY, MD ;

SCHERMAN, D ;

DEMENEIX, B ;

BEHR, JP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) :7297-7301

[3] Tumor-targeted delivery of siRNA by non-viral vector: safe and effective cancer therapy [J].

Chen, Yunching ;

Huang, Leaf .

EXPERT OPINION ON DRUG DELIVERY, 2008, 5 (12) :1301-1311

[4] Efficient Intracellular Gene Delivery Using the Formulation Composed of Poly (L-glutamic Acid) Grafted Polyethylenimine and Histone [J].

Deng, Jingjing ;

Wen, Yuting ;

Wang, Cuifeng ;

Pan, Shirong ;

Gu, Huaiyu ;

Zeng, Xin ;

Han, Lina ;

Zhao, Yuefang ;

Feng, Min ;

Wu, Chuanbin .

PHARMACEUTICAL RESEARCH, 2011, 28 (04) :812-826

[5] A Serum-Tolerant Hydroxyl-Modified Polyethylenimine as Versatile Carriers of pDNA/siRNA [J].

Dong, Xuan ;

Lin, Lin ;

Chen, Jie ;

Guo, Zhaopei ;

Tian, Huayu ;

Li, Yuce ;

Wei, Yen ;

Chen, Xuesi .

MACROMOLECULAR BIOSCIENCE, 2013, 13 (04) :512-522

[6] A hyperthermostable bacterial histone-like protein as an efficient mediator for transfection of eukaryotic cells [J].

Esser, D ;

Amanuma, H ;

Yoshiki, A ;

Kusakabe, M ;

Rudolph, R ;

Böhm, G .

NATURE BIOTECHNOLOGY, 2000, 18 (11) :1211-1213

[7] In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. [J].

Fischer, D ;

Li, YX ;

Ahlemeyer, B ;

Krieglstein, J ;

Kissel, T .

BIOMATERIALS, 2003, 24 (07) :1121-1131

[8] Inhibition of lung tumor growth by complex pulmonary delivery of drugs with oligonucleotides as suppressors of cellular resistance [J].

Garbuzenko, Olga B. ;

Saad, Maha ;

Pozharov, Vitaly P. ;

Reuhl, Kenneth R. ;

Mainelis, Gediminas ;

Minko, Tamara .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (23) :10737-10742

[9] High-affinity DNA binding of HU protein from the hyperthermophile Thermotoga maritima [J].

Grove, A ;

Lim, L .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 311 (03) :491-502

[10] Genipin-Cross-Linked Thermophilic Histone-Polyethylenimine as a Hybrid Gene Carrier [J].

Han, Haobo ;

Shi, Hui ;

Wu, Di ;

Li, Chunjie ;

Zhang, Yan ;

Xing, Zhen ;

Shi, Wei ;

Li, Quanshun .

ACS MACRO LETTERS, 2015, 4 (05) :575-578