The Winding Pathway to Erythropoietin Along the Chemistry-Biology Frontier: A Success At Last

被引：40
作者：
Wilson, Rebecca M. [1 ]
Dong, Suwei [1 ]
Wang, Ping [1 ]
Danishefsky, Samuel J. [1 ,2 ]
机构：
[1] Sloan Kettering Inst Canc Res, Bioorgan Chem Lab, New York, NY 10065 USA
[2] Columbia Univ, Dept Chem, New York, NY 10027 USA
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 30期
关键词：
amino acids; erythropoietin; glycoprotein; synthetic methods; total synthesis; NATIVE CHEMICAL LIGATION; HUMAN PARATHYROID-HORMONE; BUILDING-BLOCKS; POLYPEPTIDE-SYNTHESIS; GLYCOPEPTIDE DOMAIN; PEPTIDE; PROTEIN; GLYCOPROTEIN; CYSTEINE; COMPLEX;
D O I：
10.1002/anie.201301666
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The total synthesis of a homogeneous erythropoietin (EPO), possessing the native amino acid sequence and chitobiose glycans at each of the three wild-type sites of Nglycosylation, has been accomplished in our laboratory. We provide herein an account of our decade-long research effort en route to this formidable target compound. The optimization of the synergy of the two bedrock sciences we now call biology and chemistry was central to the success of the synthesis of EPO.
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页码：7646 / 7665
页数：20
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