Two-photon-induced fluorescence from the phycoerythrin protein

被引：24

作者：

Chen, ZP

Kaplan, DL

Yang, K

Kumar, J

Marx, KA

Tripathy, SK

机构：

[1] USA, NATICK RES DEV & ENGN CTR, DIV BIOTECHNOL, NATICK, MA 01760 USA

[2] UNIV LOWELL, DEPT CHEM, LOWELL, MA 01854 USA

[3] UNIV LOWELL, DEPT PHYS, LOWELL, MA 01854 USA

来源：

APPLIED OPTICS | 1997年 / 36卷 / 07期

关键词：

phycobiliprotein; two-photon excitation; fluorescence; biosensors;

D O I：

10.1364/AO.36.001655

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Two-photon-induced fluorescence is observed from the photodynamic phycobiliprotein phycoerythrin. Temporal, spectral, and intensity-dependent properties of the two-photon-induced fluorescence emission from phycoerythrin excited by a 1.06-mu m laser beam are reported. The measured two-photon absorption cross section of phycoerythrin is an order of magnitude larger than that of Rhodamine 6G. The potential applications of phycobiliproteins for two-photon-induced fluorescence for microscopy of three-dimensional biological samples and three-dimensional optical memory are discussed. (C) 1997 Optical Society of America.

引用

页码：1655 / 1659

页数：5

共 17 条

[1] 2-PHOTON SPECTROSCOPY OF PROTEIN-BOUND CHROMOPHORES
BIRGE, RR
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1986, 19 (05) : 138 - 146
[2] PHOTOPHYSICS AND MOLECULAR ELECTRONIC APPLICATIONS OF THE RHODOPSINS
BIRGE, RR
[J]. ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1990, 41 : 683 - 733
[3] INTERACTIONS OF PICOSECOND LASER PULSES WITH ORGANIC-MOLECULES .2. 2-PHOTON ABSORPTION CROSS-SECTIONS
BRADLEY, DJ
KOETSER, H
HUTCHINSON, MH
[J]. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1972, 329 (1576) : 105 - +
[4] THE REFRACTIVE-INDEX CORRECTION IN LUMINESCENCE SPECTROSCOPY
BUSSELLE, FJ
HAIG, ND
LEWIS, C
[J]. CHEMICAL PHYSICS LETTERS, 1980, 72 (03) : 533 - 535
[5] SOL-GEL ENCAPSULATED LIGHT-TRANSDUCING PROTEIN PHYCOERYTHRIN - A NEW BIOMATERIAL
CHEN, ZP
SAMUELSON, LA
AKKARA, J
KAPLAN, DL
GAO, H
KUMAR, J
MARX, KA
TRIPATHY, SK
[J]. CHEMISTRY OF MATERIALS, 1995, 7 (10) : 1779 - 1783
[6] CHEN ZP, 1994, MATER RES SOC SYMP P, V330, P263
[7] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY
DENK, W
STRICKLER, JH
WEBB, WW
[J]. SCIENCE, 1990, 248 (4951) : 73 - 76
[8] FLUORESCENCE OF COUMARINS AND XANTHENES AFTER 2-PHOTON ABSORPTION WITH A PULSED TITANIUM-SAPPHIRE LASER
FISCHER, A
CREMER, C
STELZER, EHK
[J]. APPLIED OPTICS, 1995, 34 (12): : 1989 - 2003
[9] PHYCOBILISOMES
GANTT, E
[J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1981, 32 : 327 - 347
[10] GLAZER AN, 1985, ANNU REV BIOPHYS BIO, V14, P47, DOI 10.1146/annurev.biophys.14.1.47

← 1 2 →