Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India

被引：22
作者：
Sumi, Vikshe [1 ]
Singh, Shambhu Dayal [1 ]
Dhama, Kuldeep [1 ]
Gowthaman, Vasudevan [1 ]
Barathidasan, Rajamani [1 ]
Sukumar, Kuppannan [2 ]
机构：
[1] Indian Vet Res Inst, Div Pathol, Avian Dis Sect, Bareilly 243122, Uttar Pradesh, India
[2] Vet Coll & Res Inst, Dept Vet Microbiol, Namakkal 637003, Tamil Nadu, India
来源：
TROPICAL ANIMAL HEALTH AND PRODUCTION | 2012年 / 44卷 / 07期
关键词：
Infectious bronchitis; 4/91; strain; Molecular characterization; S1; gene; India; POLYMERASE CHAIN-REACTION; EPIDEMIOLOGY; EVOLUTION; GENE; S1;
D O I：
10.1007/s11250-012-0140-2
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
In this study, two isolates of infectious bronchitis virus (IBV) from field outbreaks in 2008 (India/LKW/56/IVRI/08) and 2010 (India/NMK/72/IVRI/10) from broiler chickens in India were isolated and characterized. Reverse transcription polymerase chain reaction-restriction fragment length polymorphism of the entire S1 gene revealed that these isolates belong to two different genotypes, India/LKW/56/IVRI/08 as Mass strain whereas India/NMK/72/IVRI/10 as of different genotype. Nucleotide sequencing analysis showed that India/LKW/56/IVRI/08 shared 99 % homology with THA280252 (Thailand) and India/NMK/72/IVRI/10 shared greater than 99 % homology with 4/91 pathogenic strain (UK), JP/Wakayama/2/2004 (Japan) and TA03 (China), while the two Indian IBV isolates shared 73 % identity between them. Phylogenetic data allowed classification of two Indian isolates, India/LKW/56/IVRI/08 as having unique lineage within Mass genotype and India/NMK/72/IVRI/10 as of 4/91 genotype. Our study confirmed the presence of 4/91 (793/B) IBV nephropathogenic strain for the first time in India by virus isolation and sequencing.
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页码：1791 / 1795
页数：5
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