Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India

被引：22

作者：

Sumi, Vikshe ^{[1
]}

Singh, Shambhu Dayal ^{[1
]}

Dhama, Kuldeep ^{[1
]}

Gowthaman, Vasudevan ^{[1
]}

Barathidasan, Rajamani ^{[1
]}

Sukumar, Kuppannan ^{[2
]}

机构：

[1] Indian Vet Res Inst, Div Pathol, Avian Dis Sect, Bareilly 243122, Uttar Pradesh, India

[2] Vet Coll & Res Inst, Dept Vet Microbiol, Namakkal 637003, Tamil Nadu, India

来源：

TROPICAL ANIMAL HEALTH AND PRODUCTION | 2012年 / 44卷 / 07期

关键词：

Infectious bronchitis; 4/91; strain; Molecular characterization; S1; gene; India; POLYMERASE CHAIN-REACTION; EPIDEMIOLOGY; EVOLUTION; GENE; S1;

D O I：

10.1007/s11250-012-0140-2

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

In this study, two isolates of infectious bronchitis virus (IBV) from field outbreaks in 2008 (India/LKW/56/IVRI/08) and 2010 (India/NMK/72/IVRI/10) from broiler chickens in India were isolated and characterized. Reverse transcription polymerase chain reaction-restriction fragment length polymorphism of the entire S1 gene revealed that these isolates belong to two different genotypes, India/LKW/56/IVRI/08 as Mass strain whereas India/NMK/72/IVRI/10 as of different genotype. Nucleotide sequencing analysis showed that India/LKW/56/IVRI/08 shared 99 % homology with THA280252 (Thailand) and India/NMK/72/IVRI/10 shared greater than 99 % homology with 4/91 pathogenic strain (UK), JP/Wakayama/2/2004 (Japan) and TA03 (China), while the two Indian IBV isolates shared 73 % identity between them. Phylogenetic data allowed classification of two Indian isolates, India/LKW/56/IVRI/08 as having unique lineage within Mass genotype and India/NMK/72/IVRI/10 as of 4/91 genotype. Our study confirmed the presence of 4/91 (793/B) IBV nephropathogenic strain for the first time in India by virus isolation and sequencing.

引用

页码：1791 / 1795

页数：5

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