Mass Spectrometric Technology of Q Exactive for Analysis of Metabolites of Paeonol in Rat Liver Microsomes

被引：3

作者：

Guo Yan-Lei ^{[1
]}

Wu Ming-Jun ^{[1
]}

Li Wen-Juan ^{[1
]}

Jiang Zheng ^{[2
]}

Ye Fang-Ting ^{[2
]}

Yu Chao ^{[1
]}

机构：

[1] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China

[2] Thermo Fisher Sci Inc, Shanghai Demo Ctr, Shanghai 201206, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2013年 / 41卷 / 07期

关键词：

Paeonol; Resacetophenone; Rat liver microsomes; Q Exactive; MS; GLUCURONIDATION; IDENTIFICATION;

D O I：

10.3724/SP.J.1096.2013.20976

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Paeonol and resacetophenone in rat liver microsomes were analyzed by mass technology of Q exactive and the chemical structures of metabolites of paeonol and resacetophenone were analyzed in vitro by using the metabolites software of MetWorks with build-in metabolic pathways of phase I and phase II. The results suggested that 2 ',4 '-dihydroxyacetophenone was 4 '-O-demethylation metabolites of paeonol. Other metabolites such as 2 '-hydroxy-4 '-methoxybenzaldehyde and 2 ',5 '-dihydroxy-4 '-methoxyacetophenone were determined in reaction. It was also found that 2-hydroxy-O-4 '-monoglucuronideacetophenone was the principal metabolites of resacetophenone in phase.

引用

页码：1074 / 1079

页数：6

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