Ottowia beijingensis sp nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia

A taxonomic study was carried out on strain GCS-AN-3(T), which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3(T) were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5% and at temperatures from 10 to 37 degrees C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3(T) was most closely related to Ottowia pentelensis DSM 21699(T) (96.2 %). The principal fatty acids were summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c), C-16:0, summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c) and cyclo C-17:0. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3T is considered to represent a novel species of the genus Ottowia, for which the name Ottowia beijingensis sp. nov. is proposed (type strain GCS-AN-3(T)=LMG 27179(T)=CGMCC 1.12324(T)=MCCC 1A01410(T)). An emended description of the genus Ottowia is also proposed.