FORMULATION AND EVALUATION OF TRANSDERMAL PATCH FOR TREATMENT OF INFLAMMATION

Background: Quercetin is one of the important bioflavonoids present in more than twenty plants material and which is known for its anti-inflammatory, antihypertensive, vasodilator effects, antiobesity, antihyper-cholesterolemic and antiatherosclerotic activities. Free-radical is one of the key factors for the development of diseases such as hypertension, vascular disorders, and metabolic syndrome. The objective of this study was to develop a transdermal drug delivery system for Quercetin as a once-daily dosage form. Methods: Transdermal patches were prepared by solvent casting technique employing controlled release grades of HPMC and ethyl cellulose in the presence of plasticizer PEG. Standard procedures were used to analyze the prepared films for various physicochemical parameters, drug release (Franz diffusion cell) and skin irritation test. Results: The formulations were uniform in their physical characteristics with low water vapor absorption, uniformity in patch characteristics. The patches were devoid of hypersensitivity reactions on rat skin. The in-vitro release of formulation Q1, Q2, Q3, Q4, Q5 & Q6 has shown the release of about 57.02%, 52.66%, 85.77%, 74.78%, 64.27%, and 48.08% at 24 h and respectively. The order of drug release was found to be Q3>Q4>Q5> Q1>Q2>Q6. Anti-inflammatory activity by Carrageenan induced Paw edema model formulation code Q3 reduced the paw edema in a 4th hour to 0.24 +/- 0.020 which was found to be highly significant when compared to control 0.69 +/- 0.069 and standard Nu Patch 200 mg. i.e. 0.20 +/- 0.024. In Xylene induced mouse ear edema model formulation code Q3 showed 31.16 (% edema) which was found significant to when compared to controlled 88.15 (% edema).