Multicentre evaluation of Ziehl-Neelsen and light-emitting diode fluorescence microscopy in China

被引：46

作者：

Xia, H. ^{[1
]}

Song, Y-Y. ^{[1
]}

Zhao, B. ^{[1
]}

Kam, K-M. ^{[2
]}

O'Brien, R. J. ^{[3
]}

Zhang, Z-Y. ^{[4
]}

Sohn, H. ^{[5
]}

Wang, W. ^{[6
]}

Zhao, Y-L. ^{[1
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl TB Reference Lab, Natl Ctr TB Control & Prevent, Beijing 102206, Peoples R China

[2] Ctr Hlth Protect, TB Reference Lab, Publ Hlth Lab Serv Branch, Dept Hlth, Hong Kong, Hong Kong, Peoples R China

[3] Fdn Innovat New Diagnost, Geneva, Switzerland

[4] China Off, Program Appropriate Technol Hlth, Beijing, Peoples R China

[5] McGill Univ, Dept Epidemiol & Biostat, Montreal, PQ, Canada

[6] Ritsumeican Univ, Grad Sch Policy Sci, Kyoto, Japan

来源：

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2013年 / 17卷 / 01期

基金：

比尔及梅琳达.盖茨基金会;

关键词：

pulmonary tuberculosis; smear microscopy; light-emitting diode fluorescence microscopy; TUBERCULOSIS;

D O I：

10.5588/ijtld.12.0184

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

OBJECTIVE: To assess the feasibility of using light-emitting diode fluorescence microscopy (LED-FM) in peripheral laboratories in China. DESIGN: The performance of LED-FM and Ziehl-Neelsen (ZN) microscopy was compared on slides directly prepared from the sputum of tuberculosis (TB) suspects and follow-up patients on treatment. The examination time, fading of fluorescence-stained slides, average unit cost and qualitative user appraisal of LED-FM were also analysed. RESULTS: Among 11 276 slides, the smear-positive rate for LED-FM was 11.2% (1263/11276), 2.6% (294/11 276) higher than that of ZN (8.6%, 969/11276; chi(2)263.5, P < 0.05). The examination time for LED-FM (120.0 +/- 38.9 seconds) was shorter than that for ZN (206.3 +/- 75.9 s; t = 28.12, P < 0.05). For smear fading, quantitative and qualitative errors occurred within respectively 7.8 and 7.7 weeks. The average unit costs for ZN and LED-FM were respectively US$2.20 +/- 0.58 and US$1.97 +/- 0.71 (t = 5.08, P < 0.05). LED-FM was accepted by most laboratory technicians. CONCLUSION: LED-FM compared favourably with ZN, with a higher smear-positive detection rate, a shorter examination time and lower unit examination cost. LED-FM may be an alternative to ZN as a cost-effective method for detecting bacilli in peripheral laboratories in China.

引用

页码：107 / 112

页数：6

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