Biodegradation of Total Petroleum Hydrocarbons in Soil: Isolation and Characterization of Bacterial Strains from Oil Contaminated Soil

被引：14

作者：

Wang, Runkai ^{[1
,2
,3
]}

Wu, Baichun ^{[1
]}

Zheng, Jin ^{[1
]}

Chen, Hongkun ^{[1
]}

Rao, Pinhua ^{[1
,2
,3
]}

Yan, Lili ^{[2
,3
]}

Chai, Fei ^{[2
]}

机构：

[1] State Key Lab Petr Pollut Control, Beijing 102206, Peoples R China

[2] Shanghai Univ Engn Sci, Sch Chem & Chem Engn, Shanghai 201620, Peoples R China

[3] Anji Goachieve Environm Technol Co Ltd, Huzhou 313300, Peoples R China

来源：

APPLIED SCIENCES-BASEL | 2020年 / 10卷 / 12期

关键词：

total petroleum hydrocarbon; isolation; biodegradation; optimal growth condition; growth media; SP; BIOREMEDIATION; TPH;

D O I：

10.3390/app10124173

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we isolated seven strains (termedBY1-7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Following 16 rRNA sequence analysis, the strains were identified as belonging to the generaBacillus, Acinetobacter, Sphingobium, Rhodococcus,andPseudomonas,respectively. Growth characterization studies indicated that the optimal growth conditions for the majority of the strains was at 30 degrees C, with a pH value of approximately 7. Under these conditions, the strains showed a high TPH removal efficiency (50%) after incubation in beef extract peptone medium for seven days. Additionally, we investigated the effect of different growth media on growth impact factors that could potentially affect the strains' biodegradation rates. Our results suggest a potential application for these strains to facilitate the biodegradation of TPH-contaminated soil.

引用

页数：10

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