Interactions between DMPC Liposomes and the Serum Blood Proteins HSA and IgG

被引：53

作者：

Sabin, Juan ^{[2
]}

Prieto, Gerardo ^{[2
]}

Ruso, Juan M. ^{[2
]}

Messina, Paula V. ^{[3
]}

Salgado, Francisco J. ^{[4
]}

Nogueira, Montserrat ^{[4
]}

Costas, Miguel ^{[1
]}

Sarmiento, Felix ^{[2
]}

机构：

[1] Univ Nacl Autonoma Mexico, Fac Quim, Dept Fisicoquim, Lab Biofisicoquim, Mexico City 04510, DF, Mexico

[2] Univ Santiago de Compostela, Fac Fis, Dept Fis Aplicada, Grp Biofis & Interfases, Santiago De Compostela 15782, Spain

[3] Univ Nacl Sur, Dept Quim, RA-8000 Bahia Blanca, Buenos Aires, Argentina

[4] Univ Santiago de Compostela, Fac Biol, Dept Bioquim & Biol Mol, Santiago De Compostela 15782, Spain

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2009年 / 113卷 / 06期

关键词：

LIGAND-MODIFIED LIPOSOMES; INDUCED FLOCCULATION; BIOTINYLATED LIPOSOMES; PHYSICAL STABILITY; ALPHA-LACTALBUMIN; LIPID-BILAYERS; PHOSPHATIDYLCHOLINE; AGGREGATION; ALBUMIN; CONFORMATIONS;

D O I：

10.1021/jp804641e

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The interaction between two serum blood proteins, namely human serum albumin (HSA) and human immunoglobulin G (IgG), with 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC) liposomes has been studied in detail using dynamic light scattering, flow cytometry, enzyme-linked immunosorbent assay (ELISA), electrophoretic mobility, differential scanning calorimetry (DSC), and surface tension measurements. HSA and IgG interact with liposomes forming molecular aggregates that remain stable at protein concentrations beyond those of total liposome coverage. Both HSA and IgG penetrate into the liposome bilayer. An ELISA assay indicates that the Fc region of IgG is the one that is immersed in the DMPC membrane. The liposome-protein interaction is mainly of electrostatic nature, but an important hydrophobic contribution is also present.

引用

页码：1655 / 1661

页数：7

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