Combination of modified carbapenem inactivation method (mCIM) and EDTA-CIM (eCIM) for phenotypic detection of carbapenemase-producingEnterobacteriaceae

被引:53
作者
Tsai, Ya-Min [2 ]
Wang, Shining [1 ]
Chiu, Hui-Chuan [2 ]
Kao, Cheng-Yen [1 ]
Wen, Li-Li [2 ]
机构
[1] Natl Yang Ming Univ, Sch Life Sci, Inst Microbiol & Immunol, 155,Sec 2,Linong St, Taipei 112, Taiwan
[2] En Chu Kong Hosp, Dept Clin Lab, 399 Fuxing Rd, New Taipei 23702, Taiwan
关键词
Carbapenemase; Enterobacteriaceae; eCIM; mCIM; Phenotypic detection; BETA-LACTAMASE; ENTEROBACTERIACEAE; ASSAYS; HODGE;
D O I
10.1186/s12866-020-02010-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background Carbapenemase-resistantEnterobacteriaceae(CRE) cause many serious infections resulting in increasing treatment cost, prolonged hospitalization, and mortality rate. Reduced expression and/or mutations of porins and the presence of carbapenemase promoteEnterobacteriaceaesurvival under carbapenem treatments. Development of accurate methods for the detection of antimicrobial resistance is required not only for therapy but also to monitor the spread of resistant bacteria or resistance genes throughout the hospital and community. In this study, we aimed to evaluate the phenotypic methods, Modified Hodge test (MHT), modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM) for the detection of carbapenemase-producingEnterobacteriaceae(CPE). Results The results showed that mCIM had a sensitivity of 100% and a specificity of 100%, whereas the MHT had a sensitivity of 84.8% and a specificity of 97.8% for the 195 CRE isolates tested (105 CPE and 90 non-CPE isolates). The sensitivity of the mCIM/eCIM to detect metallo-carbapenemases in this study was 89.3% and the specificity was 98.7% as compared to the genotypic PCR detection. Conclusions These findings indicate that the mCIM combined with eCIM is useful for detecting and distinguishing different types of carbapenemase inEnterobacteriaceae.
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