Effect of different cellulase dosages on cell viability and ethanol production by Kluyveromyces marxianus in SSF processes

被引：41

作者：

Tomas-Pejo, E. ^{[1
]}

Garcia-Aparicio, M. ^{[1
]}

Negro, M. J. ^{[1
]}

Oliva, J. M. ^{[1
]}

Ballesteros, M. ^{[1
]}

机构：

[1] CIEMAT, Renewable Energies Dept, E-28040 Madrid, Spain

来源：

BIORESOURCE TECHNOLOGY | 2009年 / 100卷 / 02期

关键词：

Bio-ethanol; Cellulases; Inhibition; Cell growth; Kluyveromyces marxianus; SIMULTANEOUS SACCHARIFICATION; LIGNOCELLULOSIC MATERIALS; FERMENTATION; SHF; CONVERSION; GLUCOSE; YEASTS; STRAW;

D O I：

10.1016/j.biortech.2008.07.012

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

This study was aimed to study the effect of commercial cellulases (Celluclast 1.5 L FG) on Kluyveromyces marxianus CECF 10875 growth and ethanol production in SSF processes. Preliminary tests carried out in glucose (50 g/L) fermentation medium showed that high enzyme amounts (2.5-3.5 FPU/mL) could cause a negative effect on K. marxianus growth rate and viable cells number. However, the maximum ethanol production was not affected and about 86% of the theoretical (22 g/L) was reached in all cases independently of the enzyme dosage. In SSF experiments, cell viability was always affected by enzyme loading. Nervertheless, slight differences observed on cell viability during glucose fermentation processes with the detected concentrations of the additives did not justify the negative effect observed in SSF experiments. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：890 / 895

页数：6

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