Performance of the xTAG® Gastrointestinal Pathogen Panel, a Multiplex Molecular Assay for Simultaneous Detection of Bacterial, Viral, and Parasitic Causes of Infectious Gastroenteritis

被引：86

作者：

Claas, Eric C. ^{[1
]}

Burnham, Carey-Ann D. ^{[2
,3
]}

Mazzulli, Tony

Templeton, Kate ^{[4
]}

Topin, Francois ^{[5
]}

机构：

[1] Leiden Univ, Med Ctr, Dept Med Microbiol, NL-2300 RC Leiden, Netherlands

[2] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Pediat, St Louis, MO 63110 USA

[4] Univ Toronto, Mt Sinai Hosp, Univ Hlth Network, Dept Microbiol, Toronto, ON MSG 2C4, Canada

[5] Luminex BV, NL-4906 CR Oosterhout, Netherlands

来源：

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 23卷 / 07期

关键词：

Luminex; xTAG GPP; multiplex; gastroenteritis; infectious diarrhea; FECAL SAMPLES; NOROVIRUS; PCR; ADENOVIRUS; GIARDIA;

D O I：

10.4014/jmb.1212.12042

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The xTAG (R) Gastrointestinal Pathogen Panel (GPP) is a multiplexed molecular test for 15 gastrointestinal pathogens. The sensitivity and specificity of this test were assessed in 901 stool specimens collected from pediatric and adult patients at four clinical sites. A combination of conventional and molecular methods was used as comparator. Sensitivity could be determined for 12 of 15 pathogens and was 94.3% overall. The specificity across all 15 targets was 98.5%. Testing for the pathogen identified was not requested by the physician in 65% of specimens. The simultaneous detection of these 15 pathogens can provide physicians with a more comprehensive assessment of the etiology of diarrheal disease.

引用

页码：1041 / 1045

页数：5

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