A Synthetic Riboswitch that Operates using a Rationally Designed Ligand-RNA Pair

被引：16

作者：

Dohno, Chikara ^{[1
,2
]}

Kohyama, Izumi ^{[1
]}

Kimura, Maki ^{[1
]}

Hagihara, Masaki ^{[1
,3
]}

Nakatani, Kazuhiko ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

[2] PRESTO Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan

[3] Hirosaki Univ, Grad Sch Sci & Technol, Hirosaki, Aomori 0368561, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 38期

基金：

日本学术振兴会;

关键词：

naphthyridine; riboswitches; RNA-binding ligand; synthetic ligands; translational regulation; PHOTOSWITCHABLE MOLECULAR GLUE; GENE-EXPRESSION; TARGETING RNA; TRINUCLEOTIDE REPEATS; MESSENGER-RNAS; DNA; BINDING; TRANSLATION; FLEXIBILITY; INHIBITION;

D O I：

10.1002/anie.201303370

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The construction of an artificial riboswitch is based on a ligand-RNA pair without any molecular biology-based selection processes. The ligand selectively and significantly stabilized an RNA duplex containing an r(XGG)/r(XGG) sequence (X=U, A, G). The integration of the ligand-binding sequences into the 5′-untranslated region of mRNA provided an artificial riboswitch that was responsive to Z-NCTS. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9976 / 9979

页数：4

共 41 条

[1]

[Anonymous], 2012, ANGEW CHEM, V124, P3680

[2]

Chenoweth D. M., 2013, ANGEW CHEM, V125, P433

[3] Pyrrole-Imidazole Polyamides Distinguish Between Double-Helical DNA and RNA [J].

Chenoweth, David M. ;

Meier, Jordan L. ;

Dervan, Peter B. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (01) :415-418

[4] Orthogonal Riboswitches for Tuneable Coexpression in Bacteria [J].

Dixon, Neil ;

Robinson, Christopher J. ;

Geerlings, Torsten ;

Duncan, John N. ;

Drummond, Sheona P. ;

Micklefield, Jason .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2012, 51 (15) :3620-3624

[5] Reengineering orthogonally selective riboswitches [J].

Dixon, Neil ;

Duncan, John N. ;

Geerlings, Torsten ;

Dunstan, Mark S. ;

McCarthy, John E. G. ;

Leys, David ;

Micklefield, Jason .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (07) :2830-2835

[6] Photoswitchable molecular glue for DNA [J].

Dohno, Chikara ;

Uno, Shin-Nosuke ;

Nakatani, Kazuhiko .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (39) :11898-+

[7] Naphthyridine tetramer with a pre-organized structure for 1:1 binding to a CGG/CGG sequence [J].

Dohno, Chikara ;

Kohyama, Izumi ;

Hong, Changfeng ;

Nakatani, Kazuhiko .

NUCLEIC ACIDS RESEARCH, 2012, 40 (06) :2771-2781

[8] Control of DNA hybridization by photoswitchable molecular glue [J].

Dohno, Chikara ;

Nakatani, Kazuhiko .

CHEMICAL SOCIETY REVIEWS, 2011, 40 (12) :5718-5729

[9] Ligand inducible assembly of a DNA tetrahedron [J].

Dohno, Chikara ;

Atsumi, Hiroshi ;

Nakatani, Kazuhiko .

CHEMICAL COMMUNICATIONS, 2011, 47 (12) :3499-3501

[10] INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].

ELLINGTON, AD ;

SZOSTAK, JW .

NATURE, 1990, 346 (6287) :818-822

← 1 2 3 4 5 →