Comparison between 68Ga-bombesin (68Ga-BZH3) and the cRGD tetramer 68Ga-RGD4 studies in an experimental nude rat model with a neuroendocrine pancreatic tumor cell line

被引:11
作者
Cheng, Caixia [1 ]
Pan, Leyun [1 ]
Dimitrakopoulou-Strauss, Antonia [1 ]
Schaefer, Martin [2 ]
Waengler, Carmen [3 ]
Waengler, Bjoer [3 ]
Haberkorn, Uwe [1 ]
Strauss, Ludwig G. [1 ]
机构
[1] German Canc Res Ctr, Clin Cooperat Unit Nucl Med, Heidelberg, Germany
[2] German Canc Res Ctr, Dept Radiopharmaceut Chem, Heidelberg, Germany
[3] Univ Munich, Univ Hosp Munich, Dept Nucl Med, Munich, Germany
来源
EJNMMI RESEARCH | 2011年 / 1卷
关键词
Ga-68-bombesin; Ga-68-RGD tetramer; PET; kinetic modeling; neuroendocrine tumors; POSITRON-EMISSION-TOMOGRAPHY; RADIOCHEMICAL INVESTIGATIONS; INTEGRIN ALPHA-V-BETA-3; GA-68-LABELED BOMBESIN; PROSTATE-CANCER; VITRO/IN VIVO; F-18; FDG; RECEPTOR; PET; GA-68-DOTATOC;
D O I
10.1186/2191-219X-1-34
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Objectives: Receptor scintigraphy gains more interest for diagnosis and treatment of tumors, in particular for neuroendocrine tumors (NET). We used a pan-Bombesin analog, the peptide DOTA-PEG2-[D-tyr(6), beta-Ala(11), Thi(13), Nle(14)] BN(6-14) amide (BZH3). BZH3 binds to at least three receptor subtypes: the BB1 (Neuromedin B), BB2 (Gastrin-releasing peptide, GRP), and BB3. Imaging of alpha nu beta 3 integrin expression playing an important role in angiogenesis and metastasis was accomplished with a Ga-68-RGD tetramer. The purpose of this study was to investigate the kinetics and to compare both tracers in an experimental NET cell line. Methods: This study comprised nine nude rats inoculated with the pancreatic tumor cell line AR42J. Dynamic positron emission tomography (PET) scans using Ga-68-BZH3 and Ga-68-RGD tetramer were performed (Ga-68-RGD tetramer: n = 4, Ga-68-BZH3: n = 5). Standardized uptake values (SUVs) were calculated, and a two-tissue compartmental learning-machine model (calculation of K1 - k4 vessel density (VB) and receptor binding potential (RBP)) as well as a non-compartmental model based on the fractal dimension was used for quantitative analysis of both tracers. Multivariate analysis was used to evaluate the kinetic data. Results: The PET kinetic parameters showed significant differences when individual parameters were compared between groups. Significant differences were found in FD, VB, K1, and RBP (p = 0.0275, 0.05, 0.05, and 0.0275 respectively). The 56- to 60-min SUV for Ga-68-BZH3, with a range of 0.86 to 1.29 (median, 1.19) was higher than the corresponding value for the Ga-68-RGD tetramer, with a range of 0.78 to 1.31 (median, 0.99). Furthermore, FD, VB, K1, and RBP for Ga-68-BZH3 were generally higher than the corresponding values for the Ga-68-RGD tetramer, whereas k3 was slightly higher for Ga-68-RGD tetramer. Conclusions: As a parameter that reflects receptor binding, the increase of K1 for Ga-68-BZH3 indicated higher expression of bombesin receptors than that of the alpha nu beta 3 integrin in neuroendocrine tumors. Ga-68-BZH3 seems better suited for diagnosis of NETs owing to higher global tracer uptake.
引用
收藏
页码:1 / 11
页数:11
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  • [21] 2-O
  • [22] Hoffman TJ, 2003, J NUCL MED, V44, P823
  • [23] Radiometallated receptor-avid peptide conjugates for specific in vivo targeting of cancer cells
    Hoffman, TJ
    Quinn, TP
    Volkert, WA
    [J]. NUCLEAR MEDICINE AND BIOLOGY, 2001, 28 (05) : 527 - 539
  • [24] Role of integrins in cell invasion and migration
    Hood, JD
    Cheresh, DA
    [J]. NATURE REVIEWS CANCER, 2002, 2 (02) : 91 - +
  • [25] International union of pharmacology. LXVIII. Mammalian bombesin receptors: Nomenclature, distribution, pharmacology, signaling, and functions in normal and disease states
    Jensen, R. T.
    Battey, J. F.
    Spindel, E. R.
    Benya, R. V.
    [J]. PHARMACOLOGICAL REVIEWS, 2008, 60 (01) : 1 - 42
  • [26] Preparation of a promising angiogenesis PET imaging agent:: 68Ga-labeled c(RGDyK)-isothiocyanatobenzyl-1,4,7-triazacyclononane-1,4,7-triacetic acid and feasibility studies in mice
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    Lee, Yun-Sang
    Kim, Young Joo
    Cheon, Gi Jeong
    Lee, Dong Soo
    Chung, June-Key
    Lee, Myung Chul
    [J]. JOURNAL OF NUCLEAR MEDICINE, 2008, 49 (05) : 830 - 836
  • [27] The diagnosis and medical management of advanced neuroendocrine tumors
    Kaltsas, GA
    Besser, GM
    Grossman, AB
    [J]. ENDOCRINE REVIEWS, 2004, 25 (03) : 458 - 511
  • [28] Evaluation of the pharmacokinetics of 68Ga-DOTATOC in patients with metastatic neuroendocrine tumours scheduled for 90Y-DOTATOC therapy
    Koukouraki, S
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    Schuhmacher, J
    Haberkorn, U
    Karkavitsas, N
    Dimitrakopoulou-Strauss, A
    [J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2006, 33 (04) : 460 - 466
  • [29] Comparison of the pharmacokinetics of 68Ga-DOTATOC and [18F]FDG in patients with metastatic neuroendocrine tumours scheduled for 90Y-DOTATOC therapy
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    [J]. EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2006, 33 (10) : 1115 - 1122
  • [30] A JAVA']JAVA environment for medical image data analysis: initial application for brain PET quantitation
    Mikolajczyk, K
    Szabatin, M
    Rudnicki, P
    Grodzki, M
    Burger, C
    [J]. MEDICAL INFORMATICS, 1998, 23 (03): : 207 - 214