Glucose Measurement by Surface Plasmon Resonance with Borate Polymer Binding

Minimally-invasive human blood glucose detection can be realized by measuring the glucose concentration of interstitial fluid to predict the blood glucose level. As the amount of transdermally extracted interstitial fluid was minimal and its composition was complex, a glucose measurement method by surface plasmon resonance (SPR) based on PAA-ran-PAAPBA polymer binding was proposed. The polymer was immobilized on the gold film of SPR sensor using layer-by-layer self-assembly technique to capture the glucose molecules in interstitial fluid to realize the detection of glucose concentration with high precision. 2 similar to 1000mg/dL glucose solutions were measured utilizing the SPR sensor by polymer binding. The fitting degrees were 0.90177 and 0.99509 in the range of 2 similar to 10mg/dL and 25 similar to 1000mg/dL respectively. The dynamic dissociation process of glucose molecules from PAA-ran-PAAPBA was verified to be able to satisfy the requirements of the human blood glucose continuous monitoring in clinics.