E-cadherin and DAP kinase in pancreatic adenocarcinoma and corresponding lymph node metastases

被引:3
作者
Dansranjavin, T
Möbius, C
Tannapfel, A
Bartels, M
Wittekind, C
Hauss, J
Witzigmann, H
机构
[1] Univ Leipzig, Dept Surg, D-04103 Leipzig, Germany
[2] Univ Leipzig, Inst Pathol, D-04103 Leipzig, Germany
关键词
gene silencing; methylation-specific PCR; immunohistochemistry; regional lymph node metastases;
D O I
暂无
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
E-cadherin and DAP kinase have been implicated as 'invasion suppressor' genes in human cancer. The aim of this study was to analyze the methylation status of E-cadherin and DAP kinase and the expression of the protein in the metastatic lesions and to compare it with the expression in the primary tumor. Methylation-specific PCR of the DAP kinase and E-cadherin promoter was performed in 28 primary adenocarcinomas of the pancreas and in 13 corresponding regional lymph node metastases. The presence of E-cadherin and DAP kinase protein was assessed by immunohistochemistry. Metastatic lymph nodes showed a significant different expression profile from the primary tumor. E-cadherin methylation was observed in 8/28 (29%) and loss of protein expression was observed in 16/28 (57%) of pancreatic carcinomas. E-cadherin methylation was observed in 7/13 (54%) and loss of protein expression was observed in 11/13 (85%) lymph node metastases (p=0.047). DAP kinase methylation occurred in 11/28 (39%) pancreatic carcinomas and loss of protein expression was observed in 13/28 (46%). DAP kinase was methylated in 6/13 (46%) lymph node metastases and loss of protein expression was observed in 10/13 (77%) (p=0.039). Comparing primary tumor and corresponding lymph node metastases in 13 cases, the status of E-cadherin methylation was discordant in 2 cases. The protein expression pattern of E-cadherin and DAP kinase was discordant in 4 and 3 cases respectively. Unmethylated tumor samples did not express E-cadherin in 12 and DAP kinase protein in 6 cases. Our results demonstrate that reduction of E-cadherin and DAP kinase expression is more frequent in lymph node metastases than in the primary tumor and methylation of the promoter region contributes to this reduction; however, an alternative mechanism of inactivation seems to exist.
引用
收藏
页码:1125 / 1131
页数:7
相关论文
共 50 条
  • [21] Expression of E-Cadherin, β-Catenin, and CD-44v6 cell adhesion molecules in Primary Tumors and Metastases of Colorectal Adenocarcinoma
    V. V. Delektorskaya
    A. G. Perevoshchikov
    D. A. Golovkov
    N. E. Kushlinskii
    Bulletin of Experimental Biology and Medicine, 2005, 139 : 706 - 710
  • [22] Alteration of the E-Cadherin/β-Catenin Complex Is an Independent Poor Prognostic Factor in Lung Adenocarcinoma
    Kim, Hyojin
    Yoo, Seol Bong
    Sun, Pingli
    Jin, Yan
    Jheon, Sanghoon
    Lee, Choon Taek
    Chung, Jin-Haeng
    KOREAN JOURNAL OF PATHOLOGY, 2013, 47 (01) : 44 - 51
  • [23] Pancreatic carcinoma -: Correlation between E-cadherin and α-catenin expression status and liver metastasis
    Gunji, N
    Oda, T
    Todoroki, T
    Kanazawa, N
    Kawamoto, T
    Yuzawa, K
    Scarpa, A
    Fukao, K
    CANCER, 1998, 82 (09) : 1649 - 1656
  • [24] E-cadherin expression in lymph nodes of three patients with non-Hodgkin's lymphoma
    Takubo, T
    Kanashima, H
    Terada, Y
    Shibata, H
    Aoyama, Y
    Nakamae, H
    Yamamura, R
    Shima, E
    Makita, K
    Tanaka, K
    Ohta, K
    Yamane, T
    Hino, M
    Kamitani, T
    Tatsumi, N
    Ohshima, K
    HAEMATOLOGIA, 2002, 32 (01) : 67 - 72
  • [25] Immunohistochemical Analysis of E-Cadherin and Zeste Homolog 2 Expression in Endoscopic Ultrasound-Guided Fine-Needle Aspiration of Pancreatic Adenocarcinoma
    Gao, Ling
    Antic, Tatjana
    Hyjek, Elizabeth
    Gong, Can
    Mueller, Jeffrey
    Waxman, Irving
    DeMay, Richard M.
    Reeves, Ward
    CANCER CYTOPATHOLOGY, 2013, 121 (11) : 644 - 652
  • [26] E-cadherin and calretinin: a useful combination of immunochemical markers for differentiation between mesothelioma and metastatic adenocarcinoma
    Leers, MPG
    Aarts, MMJ
    Theunissen, PHMH
    HISTOPATHOLOGY, 1998, 32 (03) : 209 - 216
  • [27] The expression of EGFL7 and E-Cadherin in human adenocarcinoma of esophagogastric junction and their clinical significance
    Wang, Yun-Liang
    Xu, Song-Tao
    Shen, Xiao-Chun
    Li, Zhi
    Zhao, Xin
    Zhou, Jin
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2016, 9 (03): : 6421 - 6428
  • [28] Quantitative immunohistochemical analyses of the expression of E-cadherin, thrombomodulin, CD44H and CD44v6 in primary tumours of pharynx/larynx squamous cell carcinoma and their lymph node metastases
    Gaspar, RH
    de los Toyos, JR
    Marcos, CA
    Riera, JR
    Sampedro, A
    ANALYTICAL CELLULAR PATHOLOGY, 1999, 18 (04): : 183 - 190
  • [29] Decreased expression of E-cadherin and Yamamoto-Kohama's mode of invasion highly correlates with lymph node metastasis in esophageal squamous cell carcinoma
    Kaihara, T
    Kusaka, T
    Kawamata, H
    Oda, Y
    Fujii, S
    Morita, K
    Imura, J
    Fujimori, T
    PATHOBIOLOGY, 2001, 69 (03) : 172 - 178
  • [30] Significant correlation between micrometastasis in the lymph nodes and reduced expression of E-cadherin in early gastric cancer
    Cai J.
    Ikeguchi M.
    Tsujitani S.
    Maeta M.
    Liu J.
    Kaibara N.
    Gastric Cancer, 2001, 4 (2) : 66 - 74