Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique

被引：2

作者：

Dannhauser, David ^{[1
]}

Memmolo, Pasquale ^{[1
,2
]}

Rossi, Domenico ^{[1
]}

Merola, Francesco ^{[2
]}

Miccio, Lisa ^{[2
]}

Causa, Filippo ^{[3
]}

Ferraro, Pietro ^{[2
]}

Netti, Paolo A. ^{[1
,3
]}

机构：

[1] Ist Italiano Tecnol, Ctr Adv Biomat Healthcare CRIB, I-80125 Naples, Italy

[2] CNR, Ist Cibernet E Caianiello, I-80078 Pozzuoli, NA, Italy

[3] Univ Naples Federico II, Dipartimento Ingn Chim Mat & Prod Ind, I-80125 Naples, Italy

来源：

OPTICAL METHODS FOR INSPECTION, CHARACTERIZATION, AND IMAGING OF BIOMATERIALS II | 2015年 / 9529卷

关键词：

Light Scattering; Viscoelastic alignment; Digital holographic microscope; 3D tracking; Cell; COUPLED-DEVICE CAMERA; ERYTHROCYTE; TRACKING;

D O I：

10.1117/12.2185113

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The Light Scattering Profile (LSP) of an individual cell provides a fast and accurate characterization of its morphological properties. By combining a camera-based small angle light scattering apparatus with a microfluidic-induced particle migration technique, it is possible to characterize cells in microfluidic flows. The scattering profile of an individual cell can be fully characterized by our optimized optical light collection system. Viscoelastic-induced particle migration by polyethylene oxide implemented in a low-cost microfluidic device composed of an alignment section and a measuring section opens the possibility of precise, label-free, individual cell analysis. We have studied living cells in microfluidic flows by our light scattering apparatus and by a Digital Holographic Microscope (DHM) system. Our DHM measurements provided an accurate 3D position tracking even in multiple cell conditions.

引用

页数：6

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