Automation of Solid-Phase Extraction for Urinary Opiate Analysis

The automation of solid-phase extraction for Urinary Opiate analysis in combination with enzyme hydrolysis and derivatization in order to make the method reliable, simple and efficient was described. The extraction was performed with a RapidTrace SPE workstation using Clean Screen CSDAU SPE columns, using acetic acid, ammonium hydroxide, methanol, sodium acetate, and sodium phosphate as chemical compounds. The system consisted of reagents as methanol, distilled water, and buffers, and the samples were loaded onto the columns and allowed to pass through the sorbent. The automated system carried out one extraction in about 21 min per module, proving a reproducible, hands-free extractions, saving the analyst time. The automated extraction method also provides benefits as baseline separation of all analysts investigated, stable propyl derivatives with unique ions, also improving sensitivity and selectivity of the SPE process.