Application of FLiP Method for Differentiation of Mycobacterium tuberculosis Strains in Comparison to Commonly Used Methods, Spoligotyping and MIRU-VNTR Typing

被引：0
作者：
Zaczek, Anna [1 ]
Brzostek, Anna [2 ]
Gorna, Alina [2 ,3 ]
Sajduda, Anna [3 ]
Wojtasik, Arkadiusz [2 ]
Dziadek, Jaroslaw [1 ,2 ]
机构：
[1] Univ Rzeszow, Dept Biochem & Cell Biol, Rzeszow, Poland
[2] Polish Acad Sci, Inst Med Biol, PL-93232 Lodz, Poland
[3] Univ Lodz, Dept Genet Microorganisms, PL-90131 Lodz, Poland
来源：
POLISH JOURNAL OF MICROBIOLOGY | 2013年 / 62卷 / 01期
关键词：
Mycobacterium tuberculosis; FLiP; MIRU-VNTR typing; spoligotyping; MOLECULAR EPIDEMIOLOGY; IDENTIFICATION; MARKERS;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is 1S6110 RFLP based on 1S6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLIP), based on 1S6110 polymorphism was proposed. In this study, the discriminatory power of FLiP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis isolates was compared. The discriminatory index (HGI) of spoligotyping, MIRU-VNTR analysis, and FLiP was 0.653, 0.837, and 0.917, respectively. This indicates that FLiP allows a high level of differentiation among M. tuberculosis strains and it might be a valuable alternative to the other typing methods.
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收藏
页码：73 / 76
页数：4
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