Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

被引：51

作者：

Lorena Almaraz-Delgado, Alma ^{[1
]}

Flores-Uribe, Jose ^{[1
]}

Hugo Perez-Espana, Victor ^{[1
]}

Salgado-Manjarrez, Edgar ^{[1
]}

Agustin Badillo-Corona, Jesus ^{[1
]}

机构：

[1] Inst Politecn Nacl, Unidad Profes Interdisciplinaria Biotecnol, Mexico City 07340, DF, Mexico

来源：

AMB EXPRESS | 2014年 / 4卷

关键词：

Chlamydomonas; Chloroplast transformation; Recombinant therapeutic proteins; MOUTH-DISEASE VIRUS; SYNTHETIC BIOLOGY; EXPRESSION; TRANSFORMATION; ANTIBODY; MICROALGAE; VACCINES; PLANTS; TOOLS; ALGAE;

D O I：

10.1186/s13568-014-0057-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chloroplast transformation in the photosynthetic alga Chlamydomonas reinhardtii has been used to explore the potential to use it as an inexpensive and easily scalable system for the production of therapeutic recombinant proteins. Diverse proteins, such as bacterial and viral antigens, antibodies and, immunotoxins have been successfully expressed in the chloroplast using endogenous and chimeric promoter sequences. In some cases, proteins have accumulated to high level, demonstrating that this technology could compete with current production platforms. This review focuses on the works that have engineered the chloroplast of C. reinhardtii with the aim of producing recombinant proteins intended for therapeutical use in humans or animals.

引用

收藏

页码：1 / 9

页数：9

相关论文

共 43 条

[1] Preface - Plant-derived vaccines and antibodies: potential and limitations [J].

Arntzen, C ;

Plotkin, S ;

Dodet, B .

VACCINE, 2005, 23 (15) :1753-1756

[2] Recombinant human GAD65 accumulates to high levels in transgenic tobacco plants when expressed as an enzymatically inactive mutant [J].

Avesani, Linda ;

Vitale, Alessandro ;

Pedrazzini, Emanuela ;

deVirgilio, Maddalena ;

Pompa, Andrea ;

Barbante, Alessandra ;

Gecchele, Elisa ;

Dominici, Paola ;

Morandini, Francesca ;

Brozzetti, Annalisa ;

Falorni, Alberto ;

Pezzotti, Mario .

PLANT BIOTECHNOLOGY JOURNAL, 2010, 8 (08) :862-872

[3] Tools for chloroplast transformation in Chlamydomonas:: expression vectors and a new dominant selectable marker [J].

Bateman, JM ;

Purton, S .

MOLECULAR AND GENERAL GENETICS, 2000, 263 (03) :404-410

[4] Engineering algae for biohydrogen and biofuel production [J].

Beer, Laura L. ;

Boyd, Eric S. ;

Peters, John W. ;

Posewitz, Matthew C. .

CURRENT OPINION IN BIOTECHNOLOGY, 2009, 20 (03) :264-271

[5] Genetic engineering of the chloroplast: novel tools and new applications [J].

Bock, Ralph .

CURRENT OPINION IN BIOTECHNOLOGY, 2014, 26 :7-13

[6] Commercial production of microalgae: ponds, tanks, tubes and fermenters [J].

Borowitzka, MA .

JOURNAL OF BIOTECHNOLOGY, 1999, 70 (1-3) :313-321

[7] CHLOROPLAST TRANSFORMATION IN CHLAMYDOMONAS WITH HIGH-VELOCITY MICROPROJECTILES [J].

BOYNTON, JE ;

GILLHAM, NW ;

HARRIS, EH ;

HOSLER, JP ;

JOHNSON, AM ;

JONES, AR ;

RANDOLPHANDERSON, BL ;

ROBERTSON, D ;

KLEIN, TM ;

SHARK, KB ;

SANFORD, JC .

SCIENCE, 1988, 240 (4858) :1534-1538

[8] Protective immune response to foot-and-mouth disease virus with VP1 expressed in transgenic plants [J].

Carrillo, C ;

Wigdorovitz, A ;

Oliveros, JC ;

Zamorano, PI ;

Sadir, AM ;

Gómez, N ;

Salinas, J ;

Escribano, JM ;

Borca, MV .

JOURNAL OF VIROLOGY, 1998, 72 (02) :1688-1690

[9] Plant-made vaccine antigens and biopharmaceuticals [J].

Daniell, Henry ;

Singh, Nameirakpam D. ;

Mason, Hugh ;

Streatfield, Stephen J. .

TRENDS IN PLANT SCIENCE, 2009, 14 (12) :669-679

[10] The chloroplast transformation toolbox: selectable markers and marker removal [J].

Day, Anil ;

Goldschmidt-Clermont, Michel .

PLANT BIOTECHNOLOGY JOURNAL, 2011, 9 (05) :540-553

← 1 2 3 4 5 →