Evaluation of rat white blood cell and plasma peptidylglycine α-amidating monooxygenase activity as indicators of copper status

被引：3
作者：
Peterson, DJ [1 ]
Prohaska, JR [1 ]
机构：
[1] Univ Minnesota, Sch Med, Dept Biochem & Mol Biol, Duluth, MN 55812 USA
来源：
NUTRITION RESEARCH | 1999年 / 19卷 / 07期
关键词：
copper deficiency; rat; white blood cell; plasma; peptidylglycine; alpha-amidating monooxygenase;
D O I：
10.1016/S0271-5317(99)00064-0
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
Activity of the cuproenzyme peptidylglycine alpha-amidating monooxygenase (PAM) was studied in two-month-old male Holtzman rats following dietary copper (Cu) deficiency. Compared to Cu-adequate controls, Cu-deficient rats exhibited cardiac hypertrophy (94% increase) and lower liver Cu concentrations (87% decrease). White blood cell PAM activity was not altered by dietary Cu deficiency but activity of Cu,Zn-superoxide dismutase, another cuproenzyme, was lower in the same cells from Cu-deficient rats (68% decrease). Plasma PAM activity was lower in samples from Cu-deficient rats (32% decrease) compared to Cu-adequate rats. In additional studies it was found that PAM activity of serum was higher than plasma, that gender and type of anesthesia had little influence on PAM activity of plasma. Plasma but not white blood cell PAM activity is a useful indicator of Cu status. (C) 1999 Elsevier Science Inc.
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页码：1041 / 1047
页数：7
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