Integrated structural and functional optical imaging combining spectral-domain optical coherence and multiphoton microscopy

被引：67

作者：

Vinegoni, C

Ralston, T

Tan, W

Luo, W

Marks, DL

Boppart, SA ^{[1
]}

机构：

[1] Univ Illinois, Biophoton Imaging Lab, Beckman Inst Adv Sci & Technol, Dept Elect & Comp Engn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Bioengn, Coll Engn, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Bioengn, Coll Med, Urbana, IL 61801 USA

来源：

APPLIED PHYSICS LETTERS | 2006年 / 88卷 / 05期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1063/1.2171477

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

An integrated microscope that combines different optical techniques for simultaneous imaging is demonstrated. The microscope enables spectral-domain optical coherence microscopy based on optical backscatter, and multiphoton microscopy for the detection of two-photon fluorescence and second harmonic generation signals. The unique configuration of this integrated microscope allows for the simultaneous acquisition of both anatomical (structural) and functional imaging information with particular emphasis for applications in the fields of tissue engineering and cell biology. In addition, the contemporary analysis of the spectroscopic features can enhance contrast by differentiating among different tissue components. (c) 2006 American Institute of Physics.

引用

页码：1 / 3

页数：3

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