Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni

被引:7
作者
Goo, Youn-Kyoung [1 ,3 ]
Lee, Naeun [2 ]
Terkawi, Mohamad Alaa [1 ]
Luo, Yuzi [1 ]
Aboge, Gabriel Oluga [1 ]
Nishikawa, Yoshifumi [1 ]
Suzuki, Hiroshi [1 ]
Kim, Suk [2 ]
Xuan, Xuenan [1 ]
机构
[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan
[2] Gyeongsang Natl Univ, Coll Vet Med, Inst Anim Med, Jinju 660701, South Korea
[3] Osong Hlth Technol Adm, Div Malaria & Parasit Dis, Natl Inst Hlth, Korea CDC, Osong, South Korea
关键词
Babesia gibsoni; Immunochromatographic test; Thrombospondin-related adhesive protein; LINKED-IMMUNOSORBENT-ASSAY; ESCHERICHIA-COLI; EPIDEMIOLOGIC SURVEY; DOGS; INFECTION; EXPRESSION; IDENTIFICATION; SERODIAGNOSIS;
D O I
10.1016/j.vetpar.2012.06.024
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
We developed an immunochromatographic test (ICT) with the full-length of thrombospondin-related adhesive protein of Babesia gibsoni expressed by the modified expression method. The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:595 / 598
页数:4
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