Transferrin Adsorbed on PEGylated Gold Nanoparticles and Its Relevance to Targeting Specificity

被引：7

作者：

Cai, Chengxu ^{[1
,2
]}

Wang, Mingzhe ^{[1
,2
]}

Wang, Xinyi ^{[1
]}

Wang, Bing ^{[3
]}

Chen, Hanqing ^{[3
]}

Chen, Jian ^{[3
]}

Chen, Chunying ^{[1
]}

Feng, Weiyue ^{[3
]}

机构：

[1] Natl Ctr Nanosci & Technol, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China

来源：

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2018年 / 18卷 / 08期

关键词：

Gold Nanoparticle; Transferrin; PEGylation; Adsorption; Target Delivery; PROTEIN CORONA; POLYETHYLENE-GLYCOL; SURFACE; RECEPTOR; NANOCARRIERS; ANTIBODIES; ADSORPTION; IMPACT; CELLS;

D O I：

10.1166/jnn.2018.15435

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Polyethylene glycol (PEG) has been frequently used for surface modification of nanoparticles (NPs) to reduce non-specific binding of proteins on NPs. The investigation of protein absorption on PEGylated nanoparticles is necessary. In the work, the conjugation of transferrin (Tf) to PEGylated AuNPs via adsorption or bonding was studied. The 13 nm AuNPs were coated with various molecular weight (300, 2000, 5000) carboxyl and methoxy PEG thiol. The presence of Tf on PEGylated AuNP was characterized by dynamic light scattering (DLS) and infrared spectroscopy (IR). The data of IR confirmed the presence of Tf on PEGylated AuNPs. The diameter decrease of PEGylated AuNPs after Tf adsorption was observed by DLS measurement, which is attributed to competitive adsorption between Tf and PEG molecules. These phenomena may be important to the preservation of Tf targeting specificity on PEGylated AuNPs.

引用

页码：5306 / 5313

页数：8

共 18 条

[1] Polyethylene Glycol Backfilling Mitigates the Negative Impact of the Protein Corona on Nanoparticle Cell Targeting [J].

Dai, Qin ;

Walkey, Carl ;

Chan, Warren C. W. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (20) :5093-5096

[2] The transferrin receptor part I: Biology and targeting with cytotoxic antibodies for the treatment of cancer [J].

Daniels, Tracy R. ;

Delgado, Tracie ;

Rodriguez, Jose A. ;

Helguera, Gustavo ;

Penichet, Manuel L. .

CLINICAL IMMUNOLOGY, 2006, 121 (02) :144-158

[3] PH AND THE RECYCLING OF TRANSFERRIN DURING RECEPTOR-MEDIATED ENDOCYTOSIS [J].

DAUTRYVARSAT, A ;

CIECHANOVER, A ;

LODISH, HF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (08) :2258-2262

[4] Molecular barriers to biomaterial thrombosis by modification of surface proteins with polyethylene glycol [J].

Deible, CR ;

Petrosko, P ;

Johnson, PC ;

Beckman, EJ ;

Russell, AJ ;

Wagner, WR .

BIOMATERIALS, 1998, 19 (20) :1885-1893

[5]

FAULK WP, 1980, LANCET, V2, P390

[6]

GALBRAITH GMP, 1980, BLOOD, V55, P240

[7] IDENTIFICATION OF TRANSFERRIN RECEPTORS ON THE SURFACE OF HUMAN CULTURED-CELLS [J].

HAMILTON, TA ;

WADA, HG ;

SUSSMAN, HH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (12) :6406-6410

[8] BINDING OF APOTRANSFERRIN TO K562 CELLS - EXPLANATION OF THE TRANSFERRIN CYCLE [J].

KLAUSNER, RD ;

ASHWELL, G ;

VANRENSWOUDE, J ;

HARFORD, JB ;

BRIDGES, KR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (08) :2263-2266

[9] Lipid Layers on Nanoscale Surface Topography: Stability and Effect on Protein Adsorption [J].

Liascukiene, Irma ;

El Kirat, Karim ;

Beauvais, Mathieu ;

Asadauskas, Svajus J. ;

Lambert, Jean -Francois ;

Landoulsi, Jessem .

LANGMUIR, 2017, 33 (18) :4414-4425

[10] Impact of polymer shell on the formation and time evolution of nanoparticle-protein corona [J].

Natte, Kishore ;

Friedrich, Joerg F. ;

Wohlrab, Sebastian ;

Lutzki, Jana ;

von Klitzing, Regine ;

Oesterle, Werner ;

Orts-Gil, Guillermo .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2013, 104 :213-220

← 1 2 →