Structural alterations in rat liver proteins due to streptozotocin-induced diabetes and the recovery effect of selenium: Fourier transform infrared microspectroscopy and neural network study

被引:38
作者
Bozkurt, Ozlem [1 ]
Bayari, Sevgi Haman [2 ]
Severcan, Mete [3 ]
Krafft, Christoph [4 ]
Popp, Juergen [4 ,5 ]
Severcan, Feride [1 ]
机构
[1] Middle E Tech Univ, Dept Biol Sci, TR-06800 Ankara, Turkey
[2] Hacettepe Univ, Dept Phys Educ, Fac Educ, TR-06800 Ankara, Turkey
[3] Middle E Tech Univ, Dept Elect & Elect Engn, TR-06800 Ankara, Turkey
[4] Inst Photon Technol, D-07745 Jena, Germany
[5] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany
关键词
diabetes; liver; selenium; protein structure; Fourier transform infrared imaging; Fourier transform infrared microspectroscopy; neural networks; FTIR SPECTROSCOPIC CHARACTERIZATION; SECONDARY STRUCTURE; IR SPECTROSCOPY; MESSENGER-RNA; TISSUE; RAMAN; HEART; HIPPOCAMPUS; HOMEOSTASIS; EXPRESSION;
D O I
10.1117/1.JBO.17.7.076023
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The relation between protein structural alterations and tissue dysfunction is a major concern as protein fibrillation and/or aggregation due to structural alterations has been reported in many disease states. In the current study, Fourier transform infrared microspectroscopic imaging has been used to investigate diabetes-induced changes on protein secondary structure and macromolecular content in streptozotocin-induced diabetic rat liver. Protein secondary structural alterations were predicted using neural network approach utilizing the amide I region. Moreover, the role of selenium in the recovery of diabetes-induced alterations on macromolecular content and protein secondary structure was also studied. The results revealed that diabetes induced a decrease in lipid to protein and glycogen to protein ratios in diabetic livers. Significant alterations in protein secondary structure were observed with a decrease in alpha-helical and an increase in beta-sheet content. Both doses of selenium restored diabetes-induced changes in lipid to protein and glycogen to protein ratios. However, low-dose selenium supplementation was not sufficient to recover the effects of diabetes on protein secondary structure, while a higher dose of selenium fully restored diabetes-induced alterations in protein structure. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.7.076023]
引用
收藏
页数:8
相关论文
共 63 条
[31]  
Lin Jin-Chung, 2006, Curr Drug Discov Technol, V3, P145, DOI 10.2174/157016306778108866
[32]  
Lin SY, 2007, SPECTROSC-INT J, V21, P1
[33]   Fourier transform infrared spectroscopy used to evidence the prevention of β-sheet formation of amyloid β(1-40) peptide by a short amyloid fragment [J].
Lin, SY ;
Chu, HL .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2003, 32 (3-5) :173-177
[34]   Mechanistic Studies of Peptide Self-Assembly: Transient α-Helices to Stable β-Sheets [J].
Liu, Gai ;
Prabhakar, Anabathula ;
Aucoin, Darryl ;
Simon, Miranda ;
Sparks, Samuel ;
Robbins, Kevin J. ;
Sheen, Andrew ;
Petty, Sarah A. ;
Lazo, Noel D. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (51) :18223-18232
[35]   The Impact of Elevated Blood Glycemic Level of Patients with Type 2 Diabetes Mellitus on the Erythrocyte Membrane: FTIR Study [J].
Mahmoud, Sherif S. .
CELL BIOCHEMISTRY AND BIOPHYSICS, 2010, 58 (01) :45-51
[36]   Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis [J].
Meyer, Tobias ;
Bergner, Norbert ;
Bielecki, Christiane ;
Krafft, Christoph ;
Akimov, Denis ;
Romeike, Bernd F. M. ;
Reichart, Rupert ;
Kalff, Rolf ;
Dietzek, Benjamin ;
Popp, Juergen .
JOURNAL OF BIOMEDICAL OPTICS, 2011, 16 (02)
[37]   Beta amyloid and hyperphosphorylated tau deposits in the pancreas in type 2 diabetes [J].
Miklossy, Judith ;
Qing, Hong ;
Radenovic, Aleksandra ;
Kis, Andras ;
Vileno, Bertrand ;
Laszlo, Forro ;
Miller, Lisa ;
Martins, Ralph N. ;
Waeber, Gerard ;
Mooser, Vincent ;
Bosman, Fred ;
Khalili, Kamel ;
Darbinian, Nune ;
McGeer, Patrick L. .
NEUROBIOLOGY OF AGING, 2010, 31 (09) :1503-1515
[38]   Fourier transform infrared (FTIR) spectroscopy of biological tissues [J].
Movasaghi, Zanyar ;
Rehman, Shazza ;
Rehman, Ihtesham Ur .
APPLIED SPECTROSCOPY REVIEWS, 2008, 43 (02) :134-179
[39]   Compendium of the antidiabetic effects of supranutritional selenate doses.: In vivo and in vitro investigations with type II diabetic db/db mice [J].
Mueller, Andreas S. ;
Pallauf, Josef .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2006, 17 (08) :548-560
[40]   Selenium and diabetes: an enigma? [J].
Mueller, Andreas S. ;
Mueller, Kristin ;
Wolf, Nicole M. ;
Pallauf, Josef .
FREE RADICAL RESEARCH, 2009, 43 (11) :1029-1059