Anti-mitogenic effects of sarpogrelate in cultured rat mesangial cells

被引：8

作者：

Eto, Y ^{[1
]}

Nitta, K ^{[1
]}

Uchida, K ^{[1
]}

Tsutsui, T ^{[1
]}

Natori, K ^{[1
]}

Kawashima, A ^{[1
]}

Yumura, W ^{[1
]}

Nihei, H ^{[1
]}

机构：

[1] TOKYO WOMENS MED COLL, KIDNEY CTR, DEPT MED, SHINJUKU KU, TOKYO 162, JAPAN

来源：

LIFE SCIENCES | 1997年 / 60卷 / 11期

关键词：

sarpogrelate; serotonin; DNA synthesis; mesangial cells; cell cycle;

D O I：

10.1016/S0024-3205(97)00018-0

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We investigated the effects of the new 5-HT2A receptor antagonist, sarpogrelate, on DNA synthesis in renal mesangial cells stimulated with 5-HT in the presence and absence of platelet-derived growth factor (PDGF)-BB. Both 5-HT and PDGF-BB demonstrated a mitogenic effect on these cells. When mesangial cells were incubated in the absence of PDGF-BB, sarpogrelate inhibited DNA synthesis in these cells in a dose-dependent manner. In the presence of PDGF-BB, sarpogrelate had a weaker anti-mitogenic effect in mesangial cells stimulated with 5-HT. Sarpogrelate was cytotoxic at concentrations over 10(-5) M according to the results of LDH release assays, and it reduced the S1 phase in mesangial cells stimulated with 5-HT by a flow cytometry. These findings suggest that sarpogrelate may be effective in the treatment of some glomerulonephritis associated with mesangial cell proliferation.

引用

页码：PL193 / PL199

页数：7

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