Anti-mitogenic effects of sarpogrelate in cultured rat mesangial cells

被引：8
|
作者：
Eto, Y [1 ]
Nitta, K [1 ]
Uchida, K [1 ]
Tsutsui, T [1 ]
Natori, K [1 ]
Kawashima, A [1 ]
Yumura, W [1 ]
Nihei, H [1 ]
机构：
[1] TOKYO WOMENS MED COLL, KIDNEY CTR, DEPT MED, SHINJUKU KU, TOKYO 162, JAPAN
来源：
LIFE SCIENCES | 1997年 / 60卷 / 11期
关键词：
sarpogrelate; serotonin; DNA synthesis; mesangial cells; cell cycle;
D O I：
10.1016/S0024-3205(97)00018-0
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We investigated the effects of the new 5-HT2A receptor antagonist, sarpogrelate, on DNA synthesis in renal mesangial cells stimulated with 5-HT in the presence and absence of platelet-derived growth factor (PDGF)-BB. Both 5-HT and PDGF-BB demonstrated a mitogenic effect on these cells. When mesangial cells were incubated in the absence of PDGF-BB, sarpogrelate inhibited DNA synthesis in these cells in a dose-dependent manner. In the presence of PDGF-BB, sarpogrelate had a weaker anti-mitogenic effect in mesangial cells stimulated with 5-HT. Sarpogrelate was cytotoxic at concentrations over 10(-5) M according to the results of LDH release assays, and it reduced the S1 phase in mesangial cells stimulated with 5-HT by a flow cytometry. These findings suggest that sarpogrelate may be effective in the treatment of some glomerulonephritis associated with mesangial cell proliferation.
引用
收藏
页码：PL193 / PL199
页数：7
相关论文
共 50 条
  • [1] EFFECTS OF SARPOGRELATE ON SEROTONIN-INDUCED INCREASES IN CYTOSOLIC CA2+ IN CULTURED RAT MESANGIAL CELLS
    KANAMORI, A
    MATOBA, K
    YAJIMA, Y
    LIFE SCIENCES, 1994, 55 (18) : PL365 - PL370
  • [2] Effects of Cyclosporin A on Proliferation of Cultured Rat Mesangial Cells
    孙建平
    王韵琴
    JournalofTongjiMedicalUniversity, 1997, (02) : 115 - 117
  • [3] Effects of cyclosporin a on proliferation of cultured rat mesangial cells
    Sun Jumping
    Wang Yunqin
    Current Medical Science, 1997, 17 (2) : 115 - 117
  • [4] Effects of hypoxia on the extracellular matrix production of cultured rat mesangial cells
    Kim, SB
    Kang, SA
    Park, JS
    Lee, JS
    Hong, CD
    NEPHRON, 1996, 72 (02): : 275 - 280
  • [5] Anti-apoptotic effect of L-arginine in cultured rat mesangial cells
    Trachtman, H
    Futterweit, S
    Mermelstein, A
    Metzger, S
    Pettei, M
    INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2000, 6 (04) : 485 - 489
  • [6] THE EFFECTS OF ENDOTHELIN ON CYTOSOLIC CALCIUM IN CULTURED HUMAN AND RAT GLOMERULAR MESANGIAL CELLS
    SIMONSON, MS
    OSANAI, T
    DUNN, MJ
    CLINICAL AND INVESTIGATIVE MEDICINE-MEDECINE CLINIQUE ET EXPERIMENTALE, 1991, 14 (06): : 499 - 507
  • [7] High pressure conditions promote the proliferation of rat cultured mesangial cells in vitro
    Kawata, Y
    Fujii, Z
    Sakumura, T
    Kitano, M
    Suzuki, N
    Matsuzaki, M
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1998, 1401 (02): : 195 - 202
  • [8] Effects of Dangguibuxue decoction on rat glomerular mesangial cells cultured under high glucose conditions
    Ren, Xiao-Dan
    Zhang, Ying-Wen
    Wang, Xiu-Ping
    Li, Ya-Rong
    BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2017, 17
  • [9] Effects of Dangguibuxue decoction on rat glomerular mesangial cells cultured under high glucose conditions
    Xiao-Dan Ren
    Ying-Wen Zhang
    Xiu-Ping Wang
    Ya-Rong Li
    BMC Complementary and Alternative Medicine, 17
  • [10] Binding affinity of sarpogrelate to 5-HT2A receptor ligand recognition sites in rat renal cortical and mesangial cells in culture
    Nishio, H
    Yoshikawa, S
    Morimoto, Y
    Chen, ZQ
    Nakata, Y
    GENERAL PHARMACOLOGY, 1999, 33 (01): : 51 - 57
12345