Assessing resistance against macrocyclic lactones in gastro-intestinal nematodes in cattle using the faecal egg count reduction test and the controlled efficacy test

被引：27

作者：

De Graef, J. ^{[1
]}

Sarre, C. ^{[1
]}

Mills, B. J. ^{[2
]}

Mahabir, S. ^{[2
]}

Casaert, S. ^{[1
]}

De Wilde, N. ^{[1
]}

Van Weyenberg, M. ^{[1
]}

Geldhof, P. ^{[1
]}

Marchiondo, A. ^{[2
]}

Vercruysse, J. ^{[1
]}

Meeus, P. ^{[2
]}

Claerebout, E. ^{[1
]}

机构：

[1] Univ Ghent, Fac Vet Med, Parasitol Lab, B-9820 Merelbeke, Belgium

[2] Pfizer Anim Hlth, Kalamazoo, MI 49007 USA

来源：

VETERINARY PARASITOLOGY | 2012年 / 189卷 / 2-4期

关键词：

Ivermectin; Moxidectin; Resistance; Ostertagia ostertagi; Cooperia oncophora; Cattle; ANTHELMINTIC RESISTANCE; HAEMONCHUS-CONTORTUS; COOPERIA-ONCOPHORA; VETERINARY IMPORTANCE; OSTERTAGIA-OSTERTAGI; MOXIDECTIN; IVERMECTIN; INFECTIONS; ARGENTINA; HEALTH;

D O I：

10.1016/j.vetpar.2012.04.040

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The main objective of the present study was to evaluate the accuracy of the faecal egg count reduction test (FECRT) to assess the resistance status of ivermectin (IVM)-resistant isolates of the cattle nematodes Ostertagia ostertagi and Cooperia oncophora, using the controlled efficacy test (worm counts) as a reference. The second objective was to investigate whether both IVM-resistant isolates showed side-resistance against moxidectin (MOX) under controlled conditions. Thirty male Holstein calves Were experimentally infected with 25,000 13 of an IVM-resistant O. ostertagi isolate and 25,00013 of an IVM-resistant C. oncophora isolate. Twenty-eight days later the calves were randomly divided into 2 treatment groups and 1 untreated control group. Animals in groups 1 and 2 received MOX (Cydectin (R) 1%, Pfizer) and IVM (Ivomec (R) 1%, Merial) respectively, by subcutaneous injection at a dose rate of 0.2 mg/kg bodyweight. Faecal samples were collected 7 and 14 days after treatment and animals were necropsied 14/15 days post-treatment. Both the FECRT and the controlled efficacy test demonstrated that the O. ostertagi and C. oncophora isolates were resistant , with efficacies below 90%. The IVM-resistant O. ostertagia isolate was still susceptible to MOX treatment, as shown by over 99% reduction in egg counts and worm burden. The FECRT suggested borderline resistance against MOX in the IVM-resistant C. oncophora isolate, with egg count reductions between 97% (95% CI: 76; 100) at day 7 and 86% (95% CI: 49; 96) at day 14. However, the controlled efficacy test clearly showed MOX-resistance, with a decrease of only 31% (95% CI: 12; 57) in C. oncophora worm numbers. After MOX treatment, a significantly lower number of eggs per female C oncophora worms was counted compared to the control group (43% reduction). Due to this reduced fecundity, the FECRT may fail to detect MOX-resistance. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：378 / 382

页数：5

共 28 条

[1]

[Anonymous], 2000, NEMATODE PARASITES V

[2] A comparison of the effects of ivermectin and moxidectin on the nematode Caenorhabditis elegans [J].

Ardelli, Bernadette F. ;

Stitt, Laurel E. ;

Tompkins, Jeffrey B. ;

Prichard, Roger K. .

VETERINARY PARASITOLOGY, 2009, 165 (1-2) :96-108

[3] WORLD-ASSOCIATION-FOR-THE-ADVANCEMENT-OF-VETERINARY-PARASITOLOGY (WAAVP) METHODS FOR THE DETECTION OF ANTHELMINTIC RESISTANCE IN NEMATODES OF VETERINARY IMPORTANCE [J].

COLES, GC ;

BAUER, C ;

BORGSTEEDE, FHM ;

GEERTS, S ;

KLEI, TR ;

TAYLOR, MA ;

WALLER, PJ .

VETERINARY PARASITOLOGY, 1992, 44 (1-2) :35-44

[4] Moxidectin-resistant nematodes in cattle in Brazil [J].

Condi, G. K. ;

Soutello, R. G. V. ;

Amarante, A. F. T. .

VETERINARY PARASITOLOGY, 2009, 161 (3-4) :213-217

[5] Monitoring the efficacy of ivermectin and albendazole against gastro intestinal nematodes of cattle in Northern Europe [J].

Demeler, J. ;

Van Zeveren, A. M. J. ;

Kleinschmidt, N. ;

Vercruysse, J. ;

Hoeglund, J. ;

Koopmann, R. ;

Cabaret, J. ;

Claerebout, E. ;

Areskog, M. ;

von Samson-Himmelstjerna, G. .

VETERINARY PARASITOLOGY, 2009, 160 (1-2) :109-115

[6] Anthelmintic resistance of Ostertagia ostertagi and Cooperia oncophora to macrocyclic lactones in cattle from the western United States [J].

Edmonds, M. D. ;

Johnson, E. G. ;

Edmonds, J. D. .

VETERINARY PARASITOLOGY, 2010, 170 (3-4) :224-229

[7] Monitoring macrocyclic lactone resistance in Cooperia oncophora on a Belgian cattle farm during four consecutive years [J].

El-Abdellati, A. ;

Geldhof, P. ;

Claerebout, E. ;

Vercruysse, J. ;

Charlier, J. .

VETERINARY PARASITOLOGY, 2010, 171 (1-2) :167-171

[8] The use of a simplified faecal egg count reduction test for assessing anthelmintic efficacy on Belgian and German cattle farms [J].

El-Abdellati, A. ;

Charlier, J. ;

Geldhof, P. ;

Levecke, B. ;

Demeler, J. ;

von Samson-Himmelstjerna, G. ;

Claerebout, E. ;

Vercruysse, J. .

VETERINARY PARASITOLOGY, 2010, 169 (3-4) :352-357

[9]

Fiel CA, 2001, VET PARASITOL, V97, P211, DOI 10.1016/S0304-4017(01)00407-1

[10] Evaluation of reduction in egg shedding of gastrointestinal nematodes in cattle following administration of anthelmintics [J].

Gomes de Soutello, Ricardo Velludo ;

Dourado Coelho, Wilian Marinho ;

de Oliveira, Fernando Paes ;

Fonzar, Jose Francisco ;

Luquetti, Brenda Carla ;

Pereira de Souza, Renata Furlan ;

Zocoller Seno, Maria Conceicao ;

Talamini do Amarante, Alessandro Francisco .

REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, 2010, 19 (03) :183-185

← 1 2 3 →