Separation of multi-milligram quantities of gossypol enantiomers on polysaccharide-based stationary phases

A column (200 x 7 nun ID) of cellulose tris(3,5-dimethylphenylcarbamate) coated onto naked silica (Hypersil, particle size, 5 mum; pore size, 120 Angstrom) was used under reversed-phase condition with recycle to enantioresolve multi-milligrams quantities of gossypol enantiomers. The use of amylose derivatives and the investigation on the influence of the acidity of the supports used for the polysaccharide phases in the enantioresolution of gossypol is also discussed.