Identification of the tree shrew ATP-binding cassette transporter A1 (ABCA1) and its expression in tissues

被引：5

作者：

Zeng, Wu-Wei ^{[1
]}

Zhou, Bing ^{[1
]}

Liu, Hui-Rong ^{[1
]}

Sun, Guo-Tao ^{[1
]}

Chen, Bao-Sheng ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci,Natl Lab Med Mol Biol, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100005, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2009年 / 36卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ATP-binding cassette transporter A1 (ABCA1); cDNA sequence; High density lipoprotein (HDL); Tissue distribution; Tree shrew; DENSITY-LIPOPROTEIN DEFICIENCY; TANGIER-DISEASE; CELLULAR CHOLESTEROL; PEST SEQUENCE; APOA-I; EFFLUX; DEGRADATION; MUTATIONS; CELLS; MODEL;

D O I：

10.1007/s11033-007-9199-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP-binding cassette transporter A1 (ABCA1) modulates plasma levels of high density lipoprotein (HDL), a cardiovascular protecting factor. Tree shrew was considered to be an animal protected from atherosclerosis characterized by high proportion of HDL in plasma. The cDNA clones and expression of tree shrew ABCA1 was identified using SMART-RACE and Real-Time PCR techniques respectively. The nucleotide sequence of tree shrew ABCA1 covered 7,762 bp, including a 6,786 bp coding region which encoded a 2,261 amino acids protein with the high identity to human ABCA1 (95%). Tree shrew ABCA1 was expressed in various tissues, the highest in lung, followed by liver, kidney, spleen and cardiac muscle in turn from high to medium expression levels. This pattern was partially different from that of human ABCA1 which was low in kidney and cardiac muscle. This work could shed new light on its role of ABCA1 in the distinctive HDL metabolism in tree shrew.

引用

页码：443 / 448

页数：6

共 25 条

[1] The gene encoding ATP-binding cassette transporter 1 is mutated in Tangier disease [J].