Observation of dermal collagen fiber in wrinkled skin using polarization-resolved second-harmonic-generation microscopy

被引：42

作者：

Yasui, Takeshi ^{[1
]}

Takahashi, Yu ^{[1
]}

Fukushima, Shuichiro ^{[1
]}

Ogura, Yuki ^{[2
]}

Yamashita, Toyonobu ^{[2
]}

Kuwahara, Tomohiro ^{[2
]}

Hirao, Tetsuji ^{[2
]}

Araki, Tsutomu ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn Sci, Osaka 5608531, Japan

[2] Shiseido Res Ctr, Tsuzuki Ku, Yokohama, Kanagawa 2248558, Japan

来源：

OPTICS EXPRESS | 2009年 / 17卷 / 02期

关键词：

IN-VIVO; MULTIPHOTON FLUORESCENCE; GENERATION MICROSCOPY; CONFOCAL MICROSCOPY; HAIRLESS MOUSE; LASER-PULSES; HUMAN TISSUE; ORIENTATION; ULTRAVIOLET; DAMAGE;

D O I：

10.1364/OE.17.000912

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers. Furthermore, collagen orientation in post-UVB-exposed skin with few wrinkles changed from that of UVB-exposed wrinkled skin to that of no-UVB-exposed skin. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging. (C) 2009 Optical Society of America

引用

页码：912 / 923

页数：12

共 33 条

[1] THE OPTICS OF HUMAN-SKIN [J].