Comparison of real-time PCR and pyrosequencing for typing Bordetella pertussis toxin subunit 1 variants

被引：2

作者：

Storm, M

Advani, A

Pettersson, M

Hallander, HO

Bondeson, K ^{[1
]}

机构：

[1] Univ Uppsala Hosp, Dept Med Sci, S-75185 Uppsala, Sweden

[2] Univ Uppsala Hosp, Dept Med Sci, Uppsala, Sweden

[3] Swedish Inst Infect Dis Control, Solna, Sweden

[4] Biotage AB, Uppsala, Sweden

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 65卷 / 01期

关键词：

Bordetella pertussis; real-time PCR; pyrosequencing pertussis toxin; S1; subunit; virulence factors;

D O I：

10.1016/j.mimet.2005.07.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe two newly developed methods for rapid typing of the pertussis toxin Subunit 1 gene (ptxS1). A real-time PCR assay based on hybridization probes and a Pyrosequencing assay were developed and the specificity, sensitivity, cost, hands-on time and post-assay data processing were compared to Sanger sequencing. Both methods enabled discrimination of all four allelic variants, correctly identified all ptxS1 alleles of 143 strains tested and proved suitable for large-scale screening of B. pertussis strains. (c) 2005 Published by Elsevier B.V.

引用

收藏

页码：153 / 158

页数：6

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