Mercuration of vanillyl-alcohol oxidase from Penicillium simplicissimum generates inactive dimers

被引：21

作者：

Fraaije, MW

Mattevi, A

vanBerkel, WJH

机构：

[1] AGR UNIV WAGENINGEN,DEPT BIOCHEM,NL-6703 HA WAGENINGEN,NETHERLANDS

[2] UNIV PAVIA,DEPT GENET & MICROBIOL,I-27100 PAVIA,ITALY

来源：

FEBS LETTERS | 1997年 / 402卷 / 01期

关键词：

chemical modification; flavoprotein; heavy atom derivative; quaternary structure; vanillyl-alcohol oxidase; Penicillium simplicissimum;

D O I：

10.1016/S0014-5793(96)01494-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vanillyl-alcohol oxidase (EC 1.1.3.7) from Penicillium simplicissimum was modified with p-mercuribenzoate. One cysteine residue reacts rapidly without loss of enzyme activity. Three sulfhydryl groups then react in an 'all or none process' involving enzyme inactivation and dissociation of the octamer into dimers. The inactivation reaction is slowed down in the presence of the competitive inhibitor isoeugenol and fully reversible by treatment of the modified enzyme with dithiothreitol. Vanillyl-alcohol oxidase is more rapidly inactivated at low enzyme concentrations and protected from mercuration by antichaotropic salts. It is proposed that subunit dissociation accounts for the observed sensitivity of vanillyl-alcohol oxidase crystals towards mercury compounds.

引用

页码：33 / 35

页数：3

共 10 条

[1]

BOYER PD, 1959, ENZYMES, V1, P511

[2]

DEJONG E, 1992, EUR J BIOCHEM, V208, P651

[3] SUBSTRATE-SPECIFICITY OF FLAVIN-DEPENDENT VANILLYL-ALCOHOL OXIDASE FROM PENICILLIUM-SIMPLICISSIMUM - EVIDENCE FOR THE PRODUCTION OF 4-HYDROXYCINNAMYL ALCOHOLS FROM 4-ALLYLPHENOLS [J].