Galectin-8 Enhances T cell Response by Promotion of Antigen Internalization and Processing

被引：14

作者：

Prato, Cecilia Arahi ^{[1
]}

Carabelli, Julieta ^{[1
,2
,3
]}

Campetella, Oscar ^{[1
]}

Tribulatti, Maria Virginia ^{[1
]}

机构：

[1] Univ Nacl San Martin UNSAM, Inst Invest Biotecnol, Lab Inmunol Mol, Consejo Nacl Invest Cient & Tecn CONICET, B1650HMP, San Martin, Buenos Aires, Argentina

[2] IrsiCaixa AIDS Res Inst, Barcelona, Spain

[3] Germans Trias & Pujol Res Inst, Barcelona, Spain

来源：

ISCIENCE | 2020年 / 23卷 / 07期

关键词：

DENDRITIC CELLS; ENDOCYTOSIS; BETA-1-INTEGRINS; RECOGNITION; EXPRESSION; MIGRATION; PATHWAYS; SIGNALS; BIOLOGY; PROTEIN;

D O I：

10.1016/j.isci.2020.101278

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Galectin-8 (Gal-8) is a mammalian lectin endowed with immunostimulatory ability. In the present work, we demonstrate that Gal-8-glycan interactions on the surface of antigen-presenting cells (APCs) promote antigen binding and internalization, independently from antigen nature. Both Gal-8 and antigen were together internalized and localized in early endosomes. Interestingly, antigen processing by APCs was also accelerated in the presence of Gal-8 as a separate mechanism, distinct from the increased antigen internalization. Moreover, APCs pulsed together with antigen and Gal-8 were able to activate cognate CDC+ T cells more efficiently than those pulsed with antigen alone. This enhanced antigen presentation was still evident in the absence of costimulatory signals and APCsderived soluble mediators. Therefore, our results provide evidence for as yet unrecognized mechanism by which Gal-8 stimulates the elicitation of the immune response in a lectin-dependent manner, by inducing antigen uptake and processing upon lattice formation at APCs surface.

引用

页数：25

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